| Abstract Title |
Abstract Author |
Email |
Topic |
Abstract Body |
Co Author Name |
Authors |
Affiliations |
| Differentiation of functionally active hepatocyte-like cells from human adult bone marrow mesenchymal stem cells on a three dimentional (3D) nanofibrous scaffold |
Dr Somaieh Kazemnejad |
allameha@modares.ac.ir |
Liver Others |
Introduction: Human bone marrow derived mesenchymal stem cells (hBMSCs) have great potential for liver tissue engineering because autologous BMSCs can be harvested, expanded ex vivo, and differentiated into a hepatic phenotype for transplantation back into patient. In this study, a three dimensional (3D) nanofibrous biodegradable scaffold has been synthesized to improve the differentiation potential of hBMSCs into hepatocytes. Methods: After characterization of the MSCs derived from bone marrow they were seeded in the scaffold and induced for hepatogenic differentiation. The scaffold was prepared from a mixture of poly (å-caprolactone), polyethersulfone and collagen that mimic the natural extracellular matrix. The hepatocytes were analyzed for their cell proliferation capacity using MTT assay. Morphological, cytochemical and molecular characteristics of the hepatocytes were evaluated. Results: The proliferation rate of hepatocytes derived from stem cells was associated with increased expression of liver markers namely, albumin and á-fetoprotein, cytochrome P450 3A4, cytokeratin-18, and cytokeratin-19. Scanning electron micrograph analysis showed that the cells were well expanded on the scaffold and remained biologically and metabolically active for 21 days. Up-regulation of liver markers during differentiation was confirmed by Reveres Transcription polymerase Chain Reaction (RTPCR). The hepatocytes displayed characteristics of metabolic functions as judged by production of albumin, urea, transferrin, serum glutamic pyruvic transaminase (SGPT) and oxaloacetate aminotransferase (SGOT). Overall results indicate that the nanofibrous scaffold is useful for differentiation of functional hepatocytes derived from human BMSCs and promising for the development of a bioartificial liver system for use in patients waiting for a liver donor. |
Prof. Abdolamir Allameh |
Kazemnejad S.(1), Allameh A.(1), Soleimani M.(1), Gharehbaghian A.(2), Mohammadi Y. (3), Amirizadeh N.(2) |
(1) Faculty of Medical Science, Tarbiat Modares University, (2) Research Centre of Blood Transfusion Organization, (3) Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran. |
| LONG TERM OUTCOMES AND FACTORS PREDICTIVE OF RECURRENCE FOLLOWING ENDOSCOPIC THERAPY OF MUCOSAL ESOPHAGEAL ADENOCARCINOMA |
Dr. Prasad A Ganapathy |
prasad.ganapathy@mayo.edu |
Esophagus |
Purpose: Endoscopic therapy is an alternative to surgery in patients with mucosal (T1a) esophageal adenocarcinoma given the low likelihood of lymph nodal metastasis. We aimed to assess the long term outcomes and recurrence rates in BE patients treated endoscopically for T1a esophageal adenocarcinoma. Methods: All patients treated for T1a adenocarcinoma at Mayo Clinic Rochester between 1995 and 2007 were identified. Histology was assessed by GI pathologists. Patients were staged with EUS, CT chest/abdomen and PET scans. Patients were treated using EMR (performed with Olympus EMR cap or Wilson Cook Duette kit) and photodynamic therapy (PDT) using standard methods. Data was abstracted from a prospectively maintained database. Vital status information was queried using an institutionally approved internet research service. Results: 135 patients with mucosal adenocarcinoma were included. Mean age was 71y (SD 11), 113 (84%) were males. Mean BE length was 5.5 cm (SD 4.2). 57 (43%) received PDT. Mean follow up was 42 months (SD 32). 15 patients (13.5%) had recurrent carcinoma detected during follow up. Median time to recurrence was 16 months (IQR 10-30m). All recurrent tumors were intramucosal carcinomas: 13 managed endoscopically and 2 with esophagectomy. Patients with incident cancers, those with longer segments of BE and needing multiple treatment sessions to achieve remission, had higher risk of recurrence. Overall survival was 83% at 5 years. Cancer free survival was 79% at 5 years. Conclusion: Endoscopic therapy is a viable alternative for patients with T1a adenocarcinoma. Recurrent cancers are early stage and can be managed endoscopically with EMR. |
Dr. Prasad A Ganapathy |
Ganapathy A. Prasad, Rami J. Badreddine, Navtej S. Buttar, Louis M. Wongkeesong, Lori S. Lutzke, Lynn S. Borkenhagen, Kelly T. Dunagan, Kenneth K. Wang |
Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA |
| Spontaneous ALT flares in Asymptomatic HBeAg Negative Chronic Hepatitis B Virus Infected Patients Presenting with Normal ALT. |
Dr. Manoj Kumar Sharma |
manojkumardm@gmail.com |
Liver – Viral Hepatitis |
Background & aims: There is no information on the frequency and factors predictive of spontaneous ALT flares among asymptomatic HBeAg negative chronic Hepatitis B virus (CHBV) infected patients presenting with normal ALT from the India. Methods: 217 asymptomatic HBeAg negative/ anti-HBe positive CHBV infected patients presenting with normal ALT [ M(%): 161(74.2); Age:35.3¡À13.4 yrs; Genotypes A/C/D/A+D(%):22.6/1.4/68.7/7.4%; Precore mutation(%): 40.1% , basal core promoter mutation(%):23.5%] were prospectively followed up. Spontaneous ALT flare was diagnosed when ALT levels rose to more than twice the ULN, accompanied by HBV DNA levels of ¡Ý 5 logcopies/mL or 100 fold rise in HBVDNA from the previous levels. Results: During a median follow up of 69.0(12-144) months, spontaneous ALT flares occurred in 43 patients with an annual rate of 4.3%. The cumulative probabilities of ALT flare were 10.83% and 47.29 % respectively, after 5 and 10 years of follow-up.Multinomial logistic regression analyses showed that the age ¡Ý30 years at presentation [OR (95% CI): 5.31(1.53-18.39); P=0.008], male sex [OR(95% CI):4.54(1.01-20.76); P=0.05] and presence of precore mutation[OR(95% CI): 10.99(3.67-32.92) ;P < 0.001] were independent predictors of ALT flare. Genotype and presence of basal core promoter mutations did not predict ALT flare. Median time to spontaneous ALT flare after enrollement was 25 months [ Range:1-128 months;10th percentile:3.4 months].Conclusions: The annual rate of ALT flare of hepatitis B in asymptomatic HBeAg negative CHBV infected patients with normal ALT at presentation is estimated at 4.3%. Presence of precore mutants, male sex and age ¡Ý30 year at presentation are independent predictors of ALT flare. |
Prof Shiv Sarin |
Manoj Kumar, Ranjit Chauhan, Nitin Gupta, Syed Hissar, Puja Sakhuja, Shiv K Sarin |
Department of Gastroenterology,G.B. Pant Hospital, New Delhi- 110002, India |
| Influence of ethnicity on the outcome of patients with hepatocellular carcinoma |
Dr. Hossein Poustchi |
hossein_poustchi@wmi.usyd.edu.au |
Liver Others |
Whilst considerable variability exists in the incidence and aetiology of hepatocellular carcinoma (HCC) among different populations, no study has examined for the specific effects of ethnicity on survival. The Aim of the present study was to determine if ethnicity influences disease outcomes in patients with HCC. Methods: 629 subjects with HCC diagnosed between 1997 and 2006 at two referral centres in Sydney, Australia were prospectively studied. Results: The mean age at tumour diagnosis was 57.7±11.4 years and the majority (80%) were male. The underlying etiology for HCC in Asians (266 patients) and Caucasians (363 patients) respectively was chronic hepatitis B [CHB] (64.5% and 43%), chronic hepatitis C [CHC] (24% and 26.5%) and alcoholic liver disease (2.5% and 11.5%). The median survival was 26.6 months and five-year survival 32.4%. There were significant differences in median survival between Asians and Caucasians (32.73 vs. 22.27 months, p=0.014). The estimated 1, 3, and 5-year survival for Asians and Caucasians was 72%, 47.5%, 36.5% and 63%, 39% and 27% respectively. Analysis restricted to patients with viral hepatitis confirmed that ethnicity independently predicted better survival among Asians (p=0.048). By multiple logistic regression, predictive factors for survival included age at tumour presentation, ethnicity, disease stage, method of diagnosis, treatment and levels of albumin, GGT and AFP. Conclusion: HCC outcomes are significantly better among Asians than Caucasians. Further studies are required to verify the present findings and to determine their biologic basis. |
Dr Jacob George |
Dr.Hossein Poustchi, Prof.Simone Strasser, Prof.Geoff Farrell, Prof.Geoff McCaughan and Prof.Jacob George |
Digestive Disease Research Centre, Shartati Hospital, Medical Science/University of Tehran, Iran, AW Morrow Gastroenterology and Liver Centre, Royal Prince Alfred Hospital, Canberra Hospital and Australian National University, |
| Human Umbilical Cord Blood-derived Multipotent Stem Cells (HMSCs) Improves Glucose Homeostasis in Liver Cirrhosis Rats |
Prof. Yun Jeong Lim |
yunjeongl@yahoo.co.kr |
Miscellaneous |
Liver cirrhosis is characterized by hepatic dysfunction with extensive and usually irreversible accumulation of fibrous tissue. Recent reports have shown that mesencymal stem cells (MSCs) can differentiate into hepatocytes and facilitate the repair of injured hepatic tissue. Disturbance in glucose metabolism is a common feature in liver cirrhosis and is associated with insulin resistance. The aim of the present study is to investigate the possible mechanisms of human umbilical cord blood-derived multipotent stem cell (HMSCs) on the glucose homeostasis in the liver cirrhosis rats induced by carbon tetrachloride (CCl4) for 8 weeks. Liver cirrhosis rats showed elevated plasma levels of liver enzymes, fibrous scarring and cirrhosis on hepatic histological examination and increased glucose levels through insulin resistance. Activity in the proximal part of the insulin signaling cascade were decreased, as evinced by decreased of key enzymes such as phosphatidylinositol-3-kinase (PI 3-kinase), protein kinase B (PKB), protin kinase C (PKC), and increased glycogen synthase kinase 3 (GSK-3). We also observed that glucose-6-phosphatase (G-6-Pase) and phosphoenolpyruvate kinase (PEPCK), two hepatic enzymes involved in gluconeogenesis, were increased in cirrhosis rats. However, infusion of HMSCs significantly increased the expressions of PI 3-kinase, PKB, and PKC-¥æ about 2-3 fold whereas, the expression of GSK3 was decreased. In addition, we found that the expressions of G-6-Pase and PEPCK were strongly decreased over 40% after infusion of HMSCs (p <0.05). Taken together, our results showed that HMSCs infusion could improve insulin resistance in rats with CCl4-induced liver cirrhosis, thereby contributing to glucose homeostasis. |
Prof Joo-Ho Chung |
Kyung Hee Jung, Yun Jeong Lim, Joo-Ho Chung* and Sung-Vin Yim* |
Departments of Internal Medicine, Dongguk University International Hospital, Dongguk University College of Medicine, Goyang, Korea;*Department of Pharmacology, School of Medicine, Kyung Hee University, Seoul, Korea |
| Role of polymorphisms of CYP1A1, CYP1A2 and CYP2E1 genes in association with Helicobacter pylori in gastric carcinogenesis |
Dr. Shweta Tripathi |
udayghoshal@yahoo.co.in |
Stomach (H – Pylori) |
Background: H. pylori is expected to alter activity of cytochrome P450 (CYP), a carcinogen-activating enzyme. We studied role of CYP1A1, CYP1A2, CYP2E1 polymorphisms and H. pylori in pathogenesis of gastric neoplasm (GN). Methods: Genotyping of CYP1A1 (3801C to T), CYP1A2 (164A to C) (PCR-RFLP) and CYP2E1 (96-bp insertion, PCR) was done in 52, 62, 45 and 100 GN, non-ulcer dyspepsia (NUD), peptic ulcer (PU) and healthy controls (HC), respectively. Serum IgG anti-H. pylori antibody was used to diagnosed H. pylori infection. Results: CYP2E1 0/96 was frequent in GN [GN vs. HC: 27/52 (52%) vs. 32/100, OR 2.3 (1-5), p=0.02; GN vs. PU: 27/52 (52%) vs. 14/45 (31%), 2.3 (1-6), p=0.04; GN vs. NUD: 1.3 (0.6-3)]. CYP1A1 CT and TT [GN vs. HC: OR 1.8 (1-4)] genotypes also conferred risk but not CYP1A2. Risk conferred with respect to H. pylori (positive vs. negative) was: C2E1 [GN vs. HC: OR 2 (1-5) vs. 6 (1-25); GN vs. PU: 1.4 (0.4-4) vs. 4 (1-17)], CYP1A1 [GN vs. HC: OR 0.7 (0.3-2) vs. 2 (0.6-7); GN vs. NUD: 0.9 (0.4-3) vs. 1.3 (0.3-6); GN vs. PU: 0.8 (0.3-2) vs. 1.6 (0.4-6)] and CYP1A2 [GN vs. HC: OR 0.4 (0.1-1) vs. 2 (0.4-9); GN vs. NUD: 0.3 (0.1-1) vs. 2.3 (0.4-12); GN vs. PU: 0.8 (0.2-3) vs. 2.4 (0.5-12)]. Conclusions: CYP2E1 0/96 and CYP1A1 variant genotypes are risk factors for GN. Presence of both variant CYP genotypes and H .pylori was associated with reduced risk. |
Dr Uday Ghoshal |
Shweta Tripathi, Ujjala Ghoshal, * Balraj Mittal, ** Neeraj Kumari, $Narendra Krishnani, $Uday C Ghoshal |
Departments of Gastroenterology, *Microbiology, **Genetics and $Pathology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow -226014, India |
| Risk of Glandular Atrophy, Intestinal Metaplasia and Dysplasia in subjects with vacA positive and complete or disrupted cagE, cagT Helicobacter pylori infection |
Dr. Santosh K. Tiwari |
tiwarimicro@yahoo.com |
Stomach (H – Pylori) |
Introduction: Glandular atrophy, Intestinal metaplasia and dysplasia are pre-neoplastic lesions common in patients with chronic gastritis as in those with H.pylori (Hp) infection. Virulent strains carrying the cag pathogenicity island (cag-PAI), vacA and early age of acquisition are the key determinants in disease development. Therefore the present study was designed to investigate the association among Hp infection with cagE, cagT and vacA genes and validate the risk of precancerous lesions in those infected with various genotypes. Methods: 120 symptomatic patients (32-55years, mean age: 38.5years) were divided into group-I, with overt gastric disorders and group-II with less intense disease. Status of cagE, cagT and vacA genes was determined by PCR and histological assessment for the presence of atrophy, IM and dysplasia was done. Results: Genotypic data revealed 42.5% strains with cagE+/cagT+/vacAs1+, and 7.5% strains with partially deleted cagE, cagT & vacAs2. With respect to the clinical status, 67.5% subjects with overt diseases were infected with cagE+/cagT+/vacAs1+ genotype whereas 22.5% NUD subjects harbored strains with partial deletions and s2 allele of vacA (p<0.01). Histology revealed atrophy, IM and dysplasia to be more prominent among subjects harboring above genotype compared to those with partially deleted genes (p<0.05). Conclusion: The results demonstrate that the risk of overt gastric diseases was progressively higher as the number of virulence genes possessed by Hp increased. In addition, strains with complete cagE, cagT and vacA s1 inflicted significant damage to the gastric mucosa and had higher predictive value for the development of pre-neoplastic lesions among younger subjects. |
Prof CH Habibullah |
Santosh K. Tiwari, Manoj G, Sivaram G, Saikant R, KS Ahmed, Zakia Abid, Md. Aejaz Habeeb, Aleem A. Khan, CM Habibullah. |
Center for Liver Research and Diagnostics, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad 500058, Andhra Pradesh, India |
| Translation Elongation Factor EEF1A2 may be a Putative Oncogene in Pancreatic Cancer by Promoting Cell Growth,Migration and Invasion |
Mr. xia hai cao |
caohaixia1981@yahoo.com.cn |
Pancreas |
Background Homo sapiens eukaryotic translation elongation factor 1 alpha 2 (EEF1A2) was recently shown to be a potential oncogene in several malignancy diseases.In present study,we examined the expression of EEF1A2 in pancreatic cancer and investigated the role of EEF1A2 in pancreatic tumor growth and progression. Methods The expression of EEF1A2 was detected in human pancreatic tumours, chronic pancreatitis, normal pancreatic tissues as well as three human pancreatic cancer cell lines.Cell proliferation, motility and adhesion were evaluated by overexpression of EEF1A2 in SW1990 cells. Effect of EEF1A2 on xenograft model of nude mice was observed by anti-PCNA and MMP-9 staining. Results There was overexpression of EEF1A2 in pancreatic tumours compared with normal pancreas and pancreatitis tissues. EEF1A2 were highly expressed in pancreatic cancer cell lines Patu8988 and BxPC-3, while little expression was detected SW1990 cells. With overexpression of EEF1A2 in SW1990 cells, the ability of cell proliferation,migration,invasion and adhesion were increased significantly (P < 0.05). Moreover,intra tumor administration of Ad5/F35-EEF1A2 significantly promoted the growth of xenograft tumors(P < 0.05). The percentage of PCNA and MMP-9 positive cells in Ad5/F35-EEF1A2 treated tumors were increased compared with control groups (P < 0.05). Conclusion EEF1A2 is overexpressed in pancreatic cancer and ectopic expression of EEF1A2 enhances tumor growth,invasion,and adhesion. EEF1A2 might provide a new diagnostic marker in pancreatic cancer and prove to be a feasible and novel target for therapeutic intervention. |
Ms haixia Cao |
Ms Haixia Cao |
shanghai ruijin hospital |
| Effects of capsaicin containing chili on gastroesophageal acid refluxes (GER) and gastric emptying (GE) in patients with gastroesophageal reflux symptoms. |
Dr. Nopavut Geratikornsupuk |
gsutep@hotmail.com |
Esophagus |
To determine the effect of chili ingestion on GER and GE in patients with GER symptoms, 11 patients with typical GERD symptoms and used to have 24 hr esophageal pH tests were included. Each underwent simultaneous esophageal pH monitoring and GE scintigraphy studies with GERD symptom evaluations after ingestion of either a standard meal(noodle soup with egg) or a spicy meal(the standard meal mixed with 2 gm chili) in random order, 1 week apart. Esophageal pH was monitored for 30 min fasting and 2 hr postprandially. Gastric scintigraphic images were acquired at evey 30 min for 2 hr postprandially. GERD was defined as the % time pH <4 at the lower esophagus >4.5%. Results: In GERD patients(N=5), spicy meals increased acid reflux numbers at the 2nd hr(16.6+/-4vs7.6+/-4)(p<0.05) after meals compared to standard meals but not at the 1st hr(10.8+/-12vs10.4+/-9). Although the esophageal acid exposure time was increased during the 2nd hr after spicy meal, there was no significant difference compared with standard meal(56+/-14%vs45+/-34%)(p>0.05). In contrast, in non-GERD patients, spicy meal produced no significant effect. The gastric retention at 60 min was greater after spicy compared to standard meals(68+/-11%vs59+/-13%)(p<0.05). The GERD symptom scores were similar(p>0.05). Conclusions: After meal ingestion, increases of the acid refluxes were sustained longer after spicy meals compared to standard meals. Spicy meal also induces more gastric retention at the 1st hr postprandial period. This study suggests that capsaicin receptors may play roles on the modulation of acid reflux numbers and GE in GERD patients. |
Dr Sutep Gonlachanvit |
Nopavut Geratikornsupuk*, Tawatchai Chaiwatanarat**, Sutep Gonlachanvit* |
*Department of Medicine, **Deoartment of Radiology, Chulalongkorn University, Bangkok, Thailand. |
| Reproducibility of gastric accommodation (GA) measurement by 99mTc-pertechnetate single-photon emission computed tomography (SPECT) in healthy volunteers |
Dr. Pertechnetate Vasavid |
gsutep@hotmail.com |
GI Motility |
To determine the reproducibility of GA measurement by 99mTc-pertechnetate SPECT, 20 healthy volunteers(9 M, age 35±9 yr) underwent 3 GA studies using SPECT, 2 times in the morning and the other in the afternoon(after 6hr fasting), on 3 separate days, at least 3 days apart, in random order. The fasting gastric scintigraphy was acquired using a triple-head gamma camera at 30 minutes after iv 99mTc-pertechnetate. Then, gastric scintigraphic images were acquired for 1 hr after a 250 ml-liquid meal(Ensure) ingestion. Gastric volumes were calculated from transaxial gastric images using Image J software. GA was the maximal postprandial volume minus fasting gastric volume. Pearson correlation, intraclass correlation coefficients(ICCs), and intra-subject coefficient of variations(CVs, expressed in percent) were calculated to assess the same-time (two morning studies), the 1st different-time (1st morning and afternoon study) and the 2nd different-time (2nd morning and afternoon study) reproducibility. Results: There were significant correlations of GA measuring at the same time (r=0.75, p<0.001), the 1st and 2nd different time (r=0.70 and 0.77, respectively, p<0.001). The GA for the same-time, the 1st different-time, and the 2nd different-time studies were 434+/-90, 432+/-57, and 447+/-57 ml(mean+/-SD), respectively. The ICCs of GA were 0.681, 0.630, 0.774, respectively, with the CVs of 9.9%, 8.1%, and 6.3%. The ICCs of the postprandial/ fasting gastric volume ratio were 0.708, 0.602, and 0.588 with the CVs of 4.3%, 13.4%, and 14.4%, respectively. Conclusions: GA measurement by 99mTc-pertechnetate SPECT is reproducible in healthy volunteers at both the same-time and the different time of the day. |
Dr Sutep Gonlachanvit |
Pataramon Vasavid*, Tawatchai Chaiwatanarat*, Sutep Gonlachanvit** |
*Department of Radiology, ** Department of Internal Medicine, Chulalongkorn University, Bangkok, Thailand |
| Higher Body Mass Index is Not Associated with Adenomatous Colorectal Polyps |
Dr. Arlinking Kho Ong |
Dr. Arlinking Kho Ong |
Dr. Arlinking Kho Ong |
Background: Obesity is associated with an increased risk of having colorectal carcinoma (CRC). However, data on the association of obesity and adenomatous polyps, precursors of CRC, are conflicting. Objective: This study aimed to determine the relationship of obesity with the prevalence of colorectal adenomatous polyps in an Asian population. Methods: A case control study was done among adult (>18 yrs old) patients undergoing colonoscopy from January 2003 to July 2007 at an Asian tertiary university hospital. Body mass index (BMI) was compared between patients with >1 adenomatous polyp and patients without polyps, CRC or inflammatory bowel disease. Results: Among 3,558 colonoscopies reviewed, 2,234 (62.8%) met inclusion criteria. Two hundred eighty three (12.67%) had adenomatous polyps while 1,951 (87.33%) had no polyps (control group). Patients with adenomatous polyps were more likely to be male (54.4% vs. 45.6%; p=0.006), older (59.7 + 13.4 vs. 50.8 + 15.6; p<0.0001) and had a trend for higher BMI (24 + 4.3 vs. 23.5 + 4.0;p=0.062) when compared to controls. However, categorization into weight classes did not reveal a higher adenomatous polyp prevalence in the overweight and obese groups (p>0.05). Multivariate analysis showed that older age (OR=7.5 (95% CI=4.5-12.5);p<0.0001) and male sex (OR=1.5 (95%CI=1.2-1.9) ;p=0.003) were the only independent predictors of having an adenomatous polyp. Conclusion: Despite a trend for higher mean BMI, Asian patients with adenomatous polyps are not more likely to be overweight or obese. Additional variables such as dietary and metabolic factors need to be identified, especially in the younger age group. |
Dr. Arlinking Kho Ong |
Arlinking Ong,M.D., Stephen Wong, M.D., Peter Andrada, M.D., Dizza Dujali, M.D., Frederick Dy, M.D., Melchor Chan, M.D., Alberto Ismael, M.D., Celina Tady, M.D., Jose Sollano Jr., M.D. |
University of Santo Tomas Hospital, Manila, Philippines |
| Epac1 activates non-CFTR Cl- channels in intestinal epithelial Cl- secretion via Rap2-PLC-Ca2+ signaling. |
Dr. Mirajul Hoque Kazi |
Dr. Mirajul Hoque Kazi |
Dr. Mirajul Hoque Kazi |
Intestinal Cl- secretion is stimulated by cAMP and [Ca2+]i. Because cAMP simultaneously activates protein kinase A (PKA) and the exchange protein directly activated by cAMP (Epac), we studied the role of Epac in cAMP stimulated Cl- secretion in T84 cells. By western blot and RT-PCR, Epac1 was found to express in T84 cells and mouse intestine. Ussing chamber experiments were done for measurement of short circuit current (Isc). The Epac agonist, 8-pCPT-2’-O-Me-cAMP stimulated Cl- secretion in T84 cells and mouse intestine which was inhibited by intracellular calcium chelator BAPTA-AM or by PLC inhibitor U73122 but not by PKA inhibitor, H-89(1mM). Moreover, 8-pCPT-2’-O-Me-cAMP increased the amount of GTP bound Rap protein and increased [Ca2+]i. The rise in [Ca2+]i by 8-pCPT-2’-O-Me-cAMP was inhibited by U73122. Apical Cl- conductance (ICl) was measured in T84 cells with basolateral membrane permeabilized by nystatin. 8-pCPT-2’-O-Me-cAMP significantly increased ICl in wild type T84 cells. This effect of 8-pCPT-2’-O-Me-cAMP was completely inhibited by BAPTA-AM but not by the CFTR Inhibitor-172 or glibenclamide, suggesting that Epac stimulated ICl was Ca2+ dependent and mediated by non-CFTR Cl- channel(s). In contrast, 8-pCPT-2’-O-Me-cAMP did not have any effect on ICl in Epac1 knock down T84 cells. There was a 50% reduction of ICl compared to that of T84WT cells by forskolin stimulation. This forskolin stimulated ICl was completely blocked by CFTR Inhibitor-172. Taken together, our results suggested that cAMP activated Cl- secretion in T84 cells via both CFTR and non-CFTR Cl- channels, the later is mediated by Epac1-Rap2-PLC-Ca2+ signaling. |
Dr. Mirajul Hoque Kazi |
Kazi Mirajul Hoque, Sandra E. Guggino and Chung-Ming Tse. |
Division of Gastroenterology, Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD-21205, USA. |
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| Genetic factor in pathogenesis of gastroesophageal reflux disease: IL1B-511*T/IL1RN*1 haplotype may protect against the disease. |
Dr. Uday C Ghoshal |
udayghoshal@yahoo.co.in |
Liver Portal Hypertension |
Background: A balance between proinflammatory cytokine IL1£] and its receptor antagonist (IL1-Ra) may influence the expression of IL1£]. IL-1£] is a potent inhibitor of gastric acid secretion and IL1RN*2 allele is associated with increased inflammatory response. These cytokines may have role in gastric acid secretion. Hence, we studied these polymorphism in GERD. Methods: One hundred twenty five patients with GERD and 368 healthy subjects (HS) were studied for IL1B-511 C/T and IL-1RN VNTR polymorphism using PCR-RFLP and PCR, respectively. Genotype frequency in patients and HS was analyzed by ƒÓ2 test; to evaluate age and gender adjusted risk of GERD [odds ratio (OR) and 95% confidence interval (CI)], logistic regression model was used. Haplotype frequencies were calculated from observed genotypes by using Arlequin software. Results: Patients with GERD (n=125, age 43.4¡Ó11.8y, 81 males) were comparable to HS (n=368, 40.9¡Ó13.7y, 238 males) in respect to age and gender. Presence of IL1B-511 CC genotype was associated with higher risk of GERD (p=0.003). IL1B-511*T carriers showed reduced risk of GERD (p=0.004). IL1RN*2 allele carriers were associated with higher risk of GERD (p=0.014, OR=1.67, 95%CI=1.1-2.53). IL1RN 1,1 genotype was lower in patients than HS (p=0.01) and IL1RN 1,2 genotype was higher in patients than HS (p=0.004). T1 (IL1B-511*T/IL1RN*1) haplotype was associated with reduced risk of GERD (p=0.041, OR=0.69, 95%CI=0.48-0.98) as shown by haplotype frequency analysis. Conclusion: IL1B-511 CC, IL1RN*2 are associated with increased risk of GERD whereas IL1B-511*T carriers and T1 haplotype (IL1B-511*T/IL1RN*1) are associated with its reduced risk. |
Prof. Uday Ghoshal |
Dipti Chourasia1, BR Achyut2, Balraj Mittal2, Shweta Tripathi1, Rama D Mittal3, Uday C Ghoshal1 |
Departments of 1Gastroenterology, 2Genetics and 3Biochemistry, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226014, India |
| The molecular mechanism of mulitidrug resistance (MDR) in gastric cancer |
Prof. Daiming Fan |
hlhyhj@126.com |
Stomach (Non H – Pylori) |
Background and Aims: MDR was the main cause for chemotherapy failure of gastric cancer. Here we aimed to investigate the molecular mechanism of MDR in gastric cancer. Methods: The drug-resistant gastric cancer cell lines were screened by stepwise selection of SGC7901 cells using vincristine, adriamycin, 5-fludrouracil and cisplatin as inducing reagents, respectively. The gene expressing profiles of multidrug-resistant gastric cancer cells compared to SGC7901 cells were analyzed by subtractive hybridization, 2-D electrophoresis, RT-PCR, western blot and immunohistochemical analysis. The roles of the differentially expressed molecules in MDR of gastric cancer were investigated using gene transfection, MTT assay, SRCA mice assay, flow cytometry assay and DNA ladder assay. The molecules-associated genes were further investigated using microarray, RT-PCR, western blot and reporter gene assay. Results: Four drug-resistant gastric cancer cell lines were established. Totally 13 molecules have been confirmed to participate in modulating the MDR of gastric cancer cells, including MGr1-Ag, RPS13, RPL23, RPL16, ZNRD1, PrPc, CacyBP, TSG101, CIAPIN1, etc. Regulation of these molecules might significantly reverse the MDR phenotype of gastric cancer cells in vitro and in vivo. The mechanisms underlying their roles in MDR of gastric cancer might be through regulation of many genes, which were related to drug accumulation, apoptosis, cell cycle, DNA synthesis and phosphorylation of protein. Conclusions: The 13 molecules might play important roles in MDR of gastric cancer, and could be considered as valuable targets for gene therapy. Further research on them might push further insight into the signal network controlling MDR of gastric cancer. |
Prof. Daiming Fan |
Daiming Fan |
Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University |
| Reverse Line Blot Hybridization for Rapid Detection of Multi-Drug Resistance in Abdominal Tuberculosis |
Dr. Sudeep Rohit Shah |
shahsudeep@hotmail.com |
Miscellaneous |
Background and Aim: Multi-drug resistant tuberculosis (MDR TB) needs rapid and accurate identification to facilitate appropriate treatment. We evaluated a Reverse Line Blot Hybridization (RLBH) assay designed and standardized in-house for the rapid detection of culture positive abdominal MDR TB. Methods: An in house RLBH membrane was prepared with 54 specific probes to detect wild type and mutant alleles of selected codons of the rpo beta, inhA, katG, rpsL and rrs genes signifying resistance to rifampicin, isoniazid and streptomycin. DNA was extracted from cultures of 29 consecutive treatment naive patients with abdominal tuberculosis and analyzed with this assay. The results were compared with antibiotic sensitivity on the TB BACTEC 460 system. Results: Of the 29 specimens of M tuberculosis, 4 showed drug resistance, one to streptomycin alone and 3 were MDR- (SIRE, SIREK and SIREEt) on culture. All these strains were successfully identified on the in-house RLBH membrane. Streptomycin resistance was due to rpsL 43A-G , rifampicin due to rpo beta 531 TTG and isoniazid due to kat G 315G-C (n=2) and inhA promoter 15C-T mutations. All these were confirmed by PCR restriction enzyme assay and sequencing. Remaining strains showed wild type alleles and were drug sensitive on culture. The RLBH assay takes 72 hours in contrast to 10 days for BACTEC culture sensitivity and 4-8 weeks for LJ medium culture sensitivity. Conclusions: The in-house designed RLBH assay rapidly and accurately detected MDR and sensitive strains of M tuberculosis isolated from patients with abdominal tuberculosis. |
Dr. Sudeep Shah |
Shubadha Shenai, Sudeep Shah, Devendra Desai, Philip Abraham, Anand Joshi, Ajita Mehta, Camilla Rodrigues |
Department of Surgery, Gastroenterology and Microbiology, PD Hinduja Hospital and MRC, Mumbai 400 016, India |
| Angiopoietin-1 and vascular endothelial growth factor synergistically promotes proliferation and inhibits apoptosis of human gastric cancer cells |
Prof. Weihao Sun |
weihaosun@hotmail.com |
Stomach (Non H – Pylori) |
Objectives: Several studies demonstrated that angiopoietin (Ang)-1 and vascular endothelial growth factor (VEGF) are critical regulators in tumor angiogenesis. The aim of this study was to investigate whether transfection with Ang-1 and VEGF into gastric cancer cells affects their biological behavior. Methods: A recombinant and replication-deficient adenoviruses encoding human Ang-1 (Ad-Ang-1) or VEGF165 (Ad-VEGF165) were constructed. The resultant Ad-Ang-1 and Ad-VEGF165 was separately or jointly transfected into human gastric cancer cells MGC803. Ang-1 and VEGF mRNA expression was examined by RT-PCR. The cell proliferation was determined by MTT assay and Ki-67 immunocytochemical stain. Apoptosis was determined by flow cytometric analysis, and the expressions of apoptosis-related Bcl-2 family genes and proteins were assessed by RT-PCR and western blot analysis respectively. Results: RT-PCR analysis showed that mRNA expression of Ang-1 and VEGF was found in the transfected MGC-803 cells. The proliferation of MGC-803 cells was significantly increased by transfection with either Ang-1 or VEGF (P < 0.05), which was paralleled with increased Ki-67 protein expression in the transfected cells (P < 0.01). Apoptosis was significantly decreased in the cells transfected with Ang-1 or VEGF as compared with the vector control and the mock control. The combined transfection with Ang-1 and VEGF165 exerted synergistic effects on proliferation promotion, apoptosis inhibition, down-regulation of Bax and up-regulation of Bcl-2 expression in MGC-803 cells. Conclusions: These findings indicate that the addition of exogenous Ang-1 and VEGF can significantly promote the proliferation of human gastric cancer cell line MGC-803 and reduce its apoptosis. |
Prof. Weihao Sun |
Prof. Weihao Sun, Mr. Xi-Long Ou, Mr. Liu Yang, Mr. Guo-Sheng Chen, Ms. Yun Shao, Prof. Guo-Xian Ding |
The First Affiliated Hospital of Nanjing Medical University, China |
| Endoscopic variceal ligation plus propranolol and isosorbide mononitrate versus endoscopic variceal ligation alone for secondary prophylaxis of variceal bleeding: A large prospective randomized controlled trial |
Dr. Ashish Kumar |
ashishk10@yahoo.com |
Liver Portal Hypertension |
Background:Both EVLand propranolol are used for secondary prophylaxis of variceal bleeding.Addition of ISMNto propranolol enhances reduction of the HVPGand improves efficacy.Combination of EVLwith the two portal pressure reducing drugs have not been studied.Methods:Patients with history of variceal bleed were randomized to EVLalone or EVLplus propranolol and ISMN(EVL+drugs).HVPG was measured at baseline.EVL was repeated every 2wk until varices were eradicated.Propranolol dose was adjusted to reduce the heart-rate to 55bpm.Dose of ISMNwas 40mg/d.Primary endpoints were rebleed or death.Secondary endpoints were complications of cirrhosis and side-effects of therapy.Results:177 patients were included(85% cirrhotics,15% non-cirrhotics).88were randomized toEVL+Drugs group and89to EVLalone group.The baseline characteristics were similar in the two groups.Ten patients were lost to follow-up(excluded from analysis).The mean doses of propranol and ISMN used were125±69mg/d and33±8mg/d respectively.During a mean follow-up of15±12months 30patients developed rebleed: 14/83(17%) in the EVL+drugs group and 16/83(19%) in the EVLgroup(p=0.842).The actuarial probability of rebleed at 1-year was 14%in the EVL+drugs group and 16%in the EVLgroup (p=0.829,log rank test).Five patients died during the study period(2 in the EVL+drugs group,3 in the EVLgroup,p=0.682).No deaths were due to variceal hemorrhage.Secondary endpoints were similar in both the groups.On univariate analysis baseline predictors of rebleed were: ascites, encephalopathy, high bilirubin, low albumin, deranged PT, high HVPG, alcohol as etiology, and high CTP score.On multivariate analysis the only significant independent risk factors for rebleed were ascites, serum albumin <3.3g/dL, and HVPG>18mmHg.Conclusions:Addition of propranolol and ISMN to EVL does not further reduce the incidence of variceal rebleeding as achieved by EVL alone.Poor liver function and high HVPG predicted rebleed. |
Dr. Shiv Kumar Sarin |
Ashish Kumar, Sanjeev Kumar Jha, Praveen Sharma, Saroj Dubey, Pankaj Tyagi, Vimal Mehta, Barjesh Chander Sharma, Shiv Kumar Sarin |
Department of Gastroenterology, G B Pant Hospital, University of Delhi, New Delhi – 110002, India |
| Rectal diclofenac reduces the risk and severity of post-ERCP pancreatitis: a prospective, randomized, double-blind controlled study |
Dr. Showkat Ali Zargar |
showkatzargar@yahoo.com |
Pancreas |
Introduction: Pancreatitis is rightly the most common and feared complication of endoscopic retrograde cholangiopancreatography and its frequency is generally about 7% to 8%. A number of drugs have been used to reduce the frequency of post-ERCP pancreatitis but with conflicting results. We assessed the whether the use of diclofenac administered as rectal suppository would reduce the frequency of post-ERCP pancreatitis. Methods: A total of 800 patients were randomly assigned in a double blind fashion to receive diclofenac given as rectal suppository of 100 mg (400 patients) or inert placebo (400 patients) 15 min before ERCP. Serum amylase levels and patient-and procedure-related risk factors for post-ERCP pancreatitis were prospectively evaluated for all patients. Results: The overall frequency of post-ERCP pancreatitis was 4.9%. Compared to placebo, diclofenac significantly reduced the frequency (6.8% vs 3%; p = 0.01) as well as severity of post-ERCP pancreatitis (placebo, 15 mild, 11 moderate, 1 severe; diclofenac, 11 mild, 1 moderate, 0 severe; p = 0.03). Conclusions: Prophylactic administration of rectal diclofenac reduces the frequency and severity of post-ERCP pancreatitis. |
Dr. Showkat Ali Zargar |
Showkat Ali Zargar, Bilal-ul Rehman, Gul Javid, Bashir Ahmad Khan, Ghulam Nabi Yattoo, Altaf Ahmad Shah, Ghulam Mohamad Gulzar, Jaswinder Singh Sodi, Mushtaq Ahmad Khan, Nisar Ahmad Shah, Hakim Mohamad Shafi, Abid Shoukat |
Department of Gastroenterology Sher-i-Kashmir Institute of Medical Sciences, Srinagar, Kashmir, India |
| Abstract Title | Abstract Author
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Topic | Abstract Body
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| Could polymorphism of alcohol metabolizing enzyme (ADH3) predispose to development of alcoholic pancreatitis? |
Ms. Divya Singh | divya_guriya@rediffmail.com |
Pancreas |
Background and aim- Why only 10-15% of chronic heavy alcohol consumers develop pancreatitis is not known. Genetic factors regulating alcohol metabolism could predispose in developing alcoholic pancreatitis (ACP). Alcohol can be metabolized by oxidative as well as non-oxidative pathways. The main oxidative pathway includes alcohol dehydrogenase (ADH) enzyme. It is speculated that polymorphism in this alcohol metabolizing enzyme gene could be associated with alcoholic pancreatitis and is the purpose of our study. Method- In this case-control study patients with tropical calcific pancreatitis (TCP) (n=40), alcoholic pancreatitis (ACP) (n=42) and healthy controls (HC) (n=70) were included. ADH3 genotyping was done by PCR-RFLP. The restriction products were analyzed on 12% polyacrylamide gel electrophoresis. Result- The frequency distribution of ADH3*1/*1 genotype was 62.5 % in ACP but in only 22.5% of TCP and 26.2 % of HC. ADH3*1/*1 genotype was significantly more frequent in ACP group in comparison to TCP (p=0.001; OR=2.77, 95% CI=1.48-5.18) and HC (p=0.01; OR=2.23, 95% CI=1.22-4.06) and was found to be associated with increased risk of alcoholic pancreatitis. The frequency of ADH3*1/*2 genotype was 27.5 %, 70 % and 71.4 % in ACP, TCP and HC group respectively. There was no statistically significant difference between the frequency distribution of ADH3*1/*1, ADH3*1/*2 and ADH3*2/*2 genotype between TCP and HC. Conclusion- This study shows that carriers of ADH3*1/*1 individuals consuming alcohol are at higher risk for alcoholic pancreatitis than those with other genotypes such as ADH3*1/*2 and ADH3*2/*2. Larger sample size is needed to confirm the findings. |
Prof. Gourdas Choudhuri |
Divya Singh, Devendra Parmar**, Eesh Bhatia*, Gourdas Choudhuri |
Department of Gastroenterology, Department of Endocrinology*, SGPGIMS, IITR**, Lucknow |
| HBV infects hepatic stellate cells and affects their proliferation and expression of collagen type |
Dr. Xuan Liu | sy_liuxuan@hotmail.com |
Liver – Viral Hepatitis |
Aims: To observe whether HBV can infect hepatic stellate cells (HSCs), and to examine the effects of HBV or HBsAg on the proliferation and collagen type I expression of HSCs. Methods: The supernatant of HepG2.2.15 cells which contained HBV-DNA or HBsAg were added into LX-2 cells for 72 hours. Cell survival was determined by MTT assay. HBV particles in LX-2 cells were detected by transmission electron microscopy. The expression of HBsAg and HBcAg were determined by confocal fluorescence microscopy. The expression levels of HBV-DNA were measured by real-time PCR. The cellular collagen type I mRNA and protein levels were quantified by reverse transcription-PCR and ELISA, respectively. Results: High concentrations of HBV (1.2~5.0¡Á105copies/ml) or HBsAg (1.25~20¦Ìg/ml) inhibited the proliferation of LX-2 cells, while low concentrations of HBV (1.0¡Á103~6.2¡Á104copies/ml) or HBsAg (0.04~0.62¦Ìg/ml) promoted the proliferation. After treating LX-2 cells with HBV for 72 hours, about 42nm HBV-sized particles and strong expression of HBsAg and HBcAg were found in the cytoplasm of LX-2 cells. HBV-DNA in the culture medium of LX-2 cells decreased at 24 hours, rose at 48 hours and thereafter, decreased again at 72 hour. The mRNA and protein expression of cellular collagen type I in LX-2 cells were significantly increased by HBV infection but not by recombinant HBsAg. Conclusions: HBV and HBsAg affect the proliferation of HSCs; HBV can transiently infect and replicate in cultured HSCs and express HBsAg and HBcAg in vitro. Furthermore, HBV can significantly increase the expression of collagen type I mRNA and protein in HSCs. |
Prof. Jidong Jia |
Xuan Liu, Shengtao Zhu, Hong You, Min Cong, Tianhui Liu, Baoen Wang, Shutian Zhang, Jidong Jia |
Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China |
| Clinical, Endoscopic and Histological Predictors for Differentiation Between Crohn’s Disease and Intestinal Tuberculosis |
Dr. Govind K Makharia |
govindmakharia@gmail.com |
Large Intestine |
Background: There is remarkable similarity in clinical, endoscopic and histological features of intestinal tuberculosis(IT) and Crohns disease(CD). Aim: To find the predictors features for differentiation of CD from IT. Methods: The data of 69 patients with CD and 68 with IT were analyzed retrospectively. CD was diagnosed as per ECCO guidelines while Paustian’s criteria with Logan's modification were used for IT. Chi square test was used to assess the associations of variables with disease. Variable showing at least moderate association were put to multivariable logistic regression analysis. Regression coefficient of final multivariate model was taken as weights for respective variables and a score for each patient was calculated. Results: In univariate analysis, 44 parameters were found to be significantly different between these 2 diseases. On multivariate analysis, blood in stool [OR 0.034 (CI 0.012-0.098)], sigmoid involvement on colonoscopy [OR 0.06 (95% CI 0.02-0.15)], crypt abnormality [OR 0.14 (0.06-0.34)] presence of lymphoid follicle [OR 0.11 (0.05-0.27)] were found to be independent predictors of CD while presence of granuloma [OR 2.09 (0.98-4.45)] predicted IT. A score was calculated viz. [Blood in stool x(-2.4) + sigmoid involvement x(-2.5) + lymphoid follicle x(-1.9) + granuloma x(2.1) + crypt abnormality x(-1.5) + 8.3]. Score of more than 6.5 favors diagnosis of IT, while less than <6.5 suggests CD with sensitivity and specificity of 81% and 87% (ROC 0.93). Conclusion: Blood in stool, involvement of sigmoid colon, presence of lymphoid follicle, crypt abnormalities and granuloma are most important features for differentiation between CD and intestinal tuberculosis. |
Dr. Govind K Makharia |
Govind K Makharia, Siddharth Srivastava, Urvashi Singh, Ashish Aggarwal, Kanika Puri, V Sreenivas, S Datta Gupta, YK Joshi |
Department of Gastroenterology and Human Nutrition, Department of Microbiology, Pathology and Biostatistics. All India Institute of Medical Sciences, New Delhi, India |
| Effects of carvedilol on collagen metabolism in rats with cholestatic hepatic fibrosis |
Prof. Xiaolan Zhang |
xiaolanzh@126.com |
Liver Others |
Objective: To investigate the effects of carvedilol on collagen metabolism in liver fibrosis rat model induced by bile duct ligation (BDL). Methods: A rat model of hepatic fibrosis was established by applying BDL. 50 male Sprague-Dawley rats were randomly divided into five groups: sham operation group, model group, small dose carvedilol group(0.1 mg·kg-1·d-1), medium dose carvedilol group(1 mg·kg-1·d-1) and large dose carvedilol group(10 mg·kg-1·d-1). All rats were sacrificed at the two week and four week respectively, HE and Masson staining were used to determine hepatic fibrosis degree. The expression of collagen I mRNA was measured by Real-time quantitative polymerase chain reaction. Western blot and Real-time Q-PCR were used to measure the dynamic expression of MMP-13 and TIMP-1 during hepatic fibrosis. Results: The pathological change of liver in the carvedilol groups was rather lighter compared with the model. Liver tissue from carvedilol groups showed marked reduction in the expression of collagen I mRNA compared with those of the model group at different timepoints. Compared to model group, MMP-13 protein and mRNA both increased in carvedilol groups. But the level of TIMP-1 protein and mRNA in carvedilol groups was both significantly lower than that in model group. Carvedilol seems to inhibit the process of hepatic fibrosis dose-dependently. Conclusion: Carvedilol has efficient actions against hepatic fibrosis induced by BDL in rats. The mechanism of the drug's action is mainly associated with adjusting of abnormal collagen metabolism, inhibiting excessive hepatic collagen production and improving degradation of the deposited collagens. |
Prof. XIAO-LAN ZHANG |
XIAO-PENG TIAN, XIAO-LAN ZHANG, LEI LIU, NA LIU, CHUN-HONG ZHAO, HUI-QING JIANG |
Department of Gastroenterology, the Second Hospital of Hebei Medical University, China |
| Epidermal growth factor induces human cervical cancer oncogene, HCCR, in gastric cells via both PI3K/Akt and MAPK pathways |
Mr. Guoxin Zhang |
guoxinz@njmu.edu.cn |
Stomach (Non H – Pylori) |
Objective Human cervical cancer oncogene (HCCR) might play a role in tumorigenesis of various human tissues. The aim of this study was to investigate the biological function of HCCR, and to determine the regulatory pathways involved in HCCR induction. Methods We transfected the human gastric epithelial cell line, GES-1 with HCCR fragment and constitutively active Akt construct, respectively, and transfected the human gastric cancer cells, SGC-7901 cells with dominant negative Akt construct. The proliferation activity was tested by both flow cytometry and MTT assay. Epidermal growth factor, EGF was used to induce HCCR expression in GES-1 and SGC-7901. The specific PI3K inhibitor, LY294002, and the specific inhibitor of MEK1, PD98059 were used to see if these pathways are involved in the HCCR induction. HCCR promoter activity was detected by reporter assay. Results Overexpression of HCCR gene promoted the proliferation of GES-1 cells. EGF could induce HCCR expression in GES-1 and SGC7901 cells in both a dose- and time-dependent manner. Pretreatment of GES-1 and SGC-7901 cells with LY294002 and PD98059 significantly suppressed EGF-induced HCCR expression, respectively. We further found that PI3K down-stream effector, Akt kinase, is related to HCCR induction. Stable transfection of constitutively active Akt caused accelerated cell division, HCCR expression and enhanced HCCR promoter activity. In contranst, dominant negative Akt slowed cell cycle progression and blocks HCCR expression. Conclusion The overexpression of HCCR gene enhances the proliferation of GES-1 cells, and the induction of HCCR was regulated by both EGF/PI3K/Akt and MAPK signaling pathway in gastric cells. |
Mr. Guoxin Zhang |
Guoxin Zhang, Yang Yang, Jing Wang, Yanmei Yang |
Department of Gastroenterology, the first affiliated hospital of Nanjing Medical Uiversity |
| The effect of gases produced by enteric bacteria on small bowel motility |
Prof. Hyojin Park |
hjpark21@yuhs.ac |
GI Motility |
Backgrounds: Of various gases produced by intestinal bacteria, it has been known the relationship between methane gas and constipation-predominant irritable bowel syndrome (IBS). And it is studied about the causal relationship between methane gas and intestinal transit time. Objectives: This study investigated the effect of hydrogen or methane that gases are produced by enteric bacteria, on small bowel motility of guinea pig. Methods: A distal ileum of guinea pig was put in peristaltic bath containing Krebs'-Henseleit (K-H) solution. The mechanical activity of the circular muscle in the guinea pig ileum was monitored using three small clips arranged at intervals of 2.5 cm. Intraluminal pressure was monitored by pressure gauge which is connected at the end of outflow tract. Mixed gas (control), hydrogen gas, and methane gas pumped (0.45 ml/min) into the lumen. Results: The peristaltic velocity was significantly delayed after gas infusion in methane group. The velocities between hydrogen and methane group were showed a significant difference. After gas infusion, the amplitude of peristaltic contraction was decreased in hydrogen group, but it was significantly increased in methane group. The amplitude and AUC of intraluminal pressure were significantly decreased after gas infusion in hydrogen and methane group. Conclusions: It suggests that methane, unlike hydrogen, augments amplitude of peristaltic wave in the small bowel but delays small bowel transit because it promotes non-propagating or segmental contractions. This study provides an experimental basis for verifying that there is significant correlation between methane producers and constipation predominant IBS. |
Prof. Hyojin Park |
Eun Ju Choi, Hyojin Park, In Soo Chung,Young Ju Lee, Sang In Lee |
Dept of Int Med. Yonsei University, College of Medicine |
| Endoscopic, 24-hour gastric and esophageal acid profile in patients with gastroesophageal reflux disease (GERD): Do patients with H. pylori infection have less severe GERD? |
Dr. Uday C Ghoshal |
udayghoshal@yahoo.co.in |
Stomach (H – Pylori) |
Background: To evaluate relationship between Helicobacter pylori (Hp) and GERD, we studied endoscopic grades of esophagitis, gastric and esophageal acid profile in patients with GRED in relation to Hp infection. Methods: Symptomatic patients (from 2005 to 2007) with GERD (diagnosed by at least two of three criteria: Carlsson-Dent score [CDS] >6, histopathology and response to proton pump inhibitor) were evaluated for GERD grading by Los Angeles (LA) classification, Hp infection by positive results in two of three tests (anti-Hp IgA ELISA, RUT and histopathology), gastric and esophageal acid exposure by 24-h dual channel pH-metry (Naik II, RedTech, USA). Results: Of 115 patients (age 40.99±13.39-y, 82 male), 53 (46.1%) had Hp infection; Hp positive and negative patients were comparable in age, gender and CDS (median 11 [1-17] vs. 11 [1-17], p=NS). Hp positive patients more often had non-erosive reflux disease (NERD) than negative group (22/53, 41.5% vs. 12/62, 19.4%, p=0.016). Among patients with ERD, LA-A was more common in Hp negative (26/62, 41.9% vs. 11/53, 20.8%, p=0.026). 75/115 underwent 24-h pH-metry. Hp negative patients had more acidic stomach than Hp positive (% time gastric pH<1.5: 4.02 [0-61.06] vs. 1.135 [0-43.56], p=0.026). Esophageal acid exposure was comparable between Hp positive and negative group (3.35 [0.02-37.68] vs. 3.69 [0-76.15], p=NS). However, in higher age (>40-y), Hp negative patients had longer reflux duration than Hp positive (12.45 [0.3-47.7]-min vs. 8.52 [0-178.8], p=0.006). Conclusion: Hp infection is associated with less severe endoscopic grades of GERD and less gastric acid secretion than without it. |
Dr. Uday C Ghoshal |
Dipti Chourasia, Asha Misra, Shweta Tripathi, Narendra Krishnani*, Uday C Ghoshal. |
Departments of Gastroenterology and *Pathology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, 226014, India. |
| A Randomized Controlled Trial Comparing The Efficacy Of Pioglitazone And Pentoxifylline In Improving The Histological And The Cytokine Profile Of Nonalcoholic Steatohepatitis (NASH) Patients. |
Mr. Ajay Kumar | drakchaudhary@yahoo.co.in |
Liver Others |
Introduction: Insulin resistance and TNF-α contribute to pathogenesis of NASH .Aim: To compare efficacy of 6-months therapy with either pentoxifylline or pioglitazone on liver histology and cytokine profile of NASH patients. Patients and Methods: 20 consecutive NASH patients [pentoxifylline(400mg.,tid),n=11, (M:F)=7:4, age(yr)=35.8±7.2, BMI(kg/m2)=23.5±1.7, pioglitazone(30mg.,o.d.),n=9, (M:F)=4:5, age(yr)=40.1±9.9, BMI(kg/m2)=26.2±3.86] with serum ALT>1.2X ULN for >6 months were enrolled. Serum adipocytokine levels and liver biopsy were evaluated before and after 6 mo of therapy. Brunt’s grade and stage of fibrosis were assessed. Results: Transaminases decreased significantly with both drugs (pentoxifylline-ALT(IU/L)=89.3±32.6vs38.45±20.6,P<0.05,AST(IU/L)=65.27±19.5vs28.5±9.7,P<0.05, pioglitazone- ALT(IU/L)=75±16.9vs26.3±16.1,P<0.05, AST(IU/L)=53.8±7.4vs24.4±7.1,P<0.05). Pentoxifylline improved HOMA(insulin resistance)(median,range) 1.9(0.82-7.7)vs1.6(0.47-4.62),P<0.05, and showed improving trends of other parameters- TNF-alfa(pg/ml)(median,range) =10.7(4-338)vs8.7(4-42),P=0.67 and adiponectin(μg/ml)(median,range) =4.5(2.2-8.0)vs5.5(3-16.0),P=0.073. Pioglitazone improved- HOMA(median,range) 3.7(1.8-5.68)vs1.77(1.48-3.49),P<0.05 and adiponectin(μg/ml)(median,range) 4.0(1.0-6.0)vs7.0(4.9-11),P<0.05 and showed improving trends of TNF-alfa(pg/ml)(median,range) 13.3(5.2-16.3)vs10.4(8.3-34.3)P=0.21. Leptin(ng/ml)(median,range) [pentoxifylline 4.48(1.75-37.91)vs23.57(3.37-52.3),P<0.05, pioglitazone13.47(6.3-64.6)vs14.38(14.2-155.1),P<0.05] significantly increased with either of drugs. Histologically, pentoxifylline significantly improved steatosis(median,range) 2(1-3)vs1(0-3),P<0.05 and showed trend towards improvements in ballooning(median,range) 1(1-2)vs1(0-2),P=0.65, lobular inflammation(median,range)1(0-2)vs1(0-2),P=0.27, portal inflammation(median,range) 1(0-2)vs1(0-2),P=0.65 and grade of activity(median,range) 1(1-3)vs1(0-3),P=0.10. Pioglitazone significantly improved- steatosis(median,range) 2(1-3)vs1(0-2),P<0.05, lobular inflammation(median,range) 1(1-2)vs0(0-1),P<0.05, portal inflammation(median,range) 2(0-2)vs0(0-1),P<0.05 and grade of activity(median,range) 2(1-3)vs1(0-2),P<0.05 and showed improving trend of ballooning(median,range) 2(1-2)vs1(0-2),P=0.15. No significant improvement in fibrosis was observed with either pentoxyfylline(median,range) 1(0-2)vs1(0-2),P=0.41 or pioglitazone(median,range) 1(0-3)vs1(0-3),P=0.38. There was no significant difference in improvement of various parameters when compared between pentoxifylline and pioglitazone. Conclusions: Both pioglitazone and pentoxifylline lead to significant improvement in transaminases, HOMA and steatosis of NASH patients. Both are equally effective in improving cytokine profile and histological markers of hepatic injury. |
Mr. Brajesh Chander Sharma |
Ajay Kumar, Abhishek Gupta, Puja Sakhuja , Barjesh Chander Sharma, Shiv Kumar Sarin |
Departments of Gastroenterology and Pathology G B Pant Hospital New Delhi, India |
| Modulation of Pacemaker Activity by Hydrogen Peroxide in Interstitial Cells of Cajal (ICC) |
Dr. Chan-Guk Park |
drcgpark@chosun.ac.kr |
GI Motility |
Background/Aims: Intestinal inflammation either in humans or in experimental animal model is associated with altered gastrointestinal motility. Several papers, in vitro studies, reported that H2O2 altered gastrointestinal smooth muscle contractility, suggest that H2O2 may be an important mediator causing dysmotility in intestinal inflammation. It has not been reported that the role of ROS on ICC activity. Therefore, we want to know that external effect of H2O2 on pacemaker activity of ICC and its signal transduction mechanisms. Methods: For our study, we prepared the cultured ICC from murine small intestine and performed the patch clamp methods. Results: H2O2 hyperpolarized the membrane using a current clamp and inhibited pacemaker currents using a voltage clamp. These effects were blocked by glibenclamide, an inhibitor of ATP-sensitive K+ (KATP) channels. The free radical scavenger enzyme catalase blocked the H2O2-induced effects. MAFP and AACOCF3 (a cytosolic phospholipase A2 inhibitors) or SC-560 and NS-398 (a selective COX-1 and 2 inhibitor) or AH6809 (an EP2 receptor antagonist) blocked the H2O2-induced effects. H2O2-induced effects could not be blocked by calphostin C (a protein kinase C inhibitor), or SQ-22536 (an adenylate cyclase inhibitor). Conclusions: These results suggest H2O2 can modulate ICC pacemaker activity and this occur by the activation of KATP channels through PGE2 production. |
Dr. Chan-Guk Park |
Chan Guk Park1, Won Kyung Jun1, Young Dae KIM1, Man Woo KIM1, Jae Yeoul JUN2, Suk Choi2 |
1Department of Internal Medicine, College of Medicine, Chosun University, 2Department of Physiology, College of Medicine, Chosun University, Gwangju, Korea |
| Assessment of small intestinal permeability using 1H-NMR spectroscopy |
Dr. Uday C Ghoshal |
udayghoshal@yahoo.co.in |
Small Intestine |
Evaluation of small intestinal permeability (SIP) is based on estimation of urinary excretion ratio of a large and a small molecule (lactulose and mannitol, L/M) after ingestion. We evaluated SIP using 1H-NMR spectroscopy. Methods: In-vitro experiments on known concentration of L/M solutions were performed to see accuracy and precession of quantification using 1H-NMR spectroscopy. Eighteen patients with malabsoption syndrome (MAS) and 28 healthy subjects (HS) underwent evaluation of SIP using L/M ratio over 6-h after ingestion of 10g lactulose and 5g mannitol using 1H-NMR spectroscopy and trimethyl silyl propionic acid as reference. Results: Median errors of estimation of mannitol and lactulose were 5% (1.2 to 5) and 1.3 % (0.2 to 1.3), respectively in-vitro. Patients with MAS excreted more of lactulose in urine than HS (median 0.33, 0 to1.09 mmol Vs 0.12, 0 to .676 mmol, p 0.008). There was a trend towards less excretion of mannitol in MAS than HS (median 3.58, range 0.61 to 15.77 mmol vs. 3.82, 1.34 to 16.42 mmol, p = ns). L/M ratio was higher in MAS as compared to HS (median 0.1172, range 0 to 0.4074 Vs 0.045, 0 to 0.259, P< 0.002). A cut-off value of L/M excretion ratio by ROC curve of 0.049 had a sensitivity and specificity of 72% and 61%, respectively; a cut-off value of 0.078 had a specificity of 90% but sensitivity was low (67%). Area under curve was 0.77. Conclusion: 1H-NMR spectroscopy is a new analytical tool for assessment of SIP with reasonable sensitivity and specificity. |
Dr. Uday C Ghoshal |
Uday C Ghoshal, K Jayalakshmi,Sunil Kumar, Asha Misra, Raja Roy, CL Khetrapal |
Department of Gastroenterology and Centre of Biomedical Magnetic Resonance, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareli Road, Lucknow-226104, India. |
| Pattern of 18F-FDG PET in cholangiocarinoma according to the tumor location and morphology |
Dr. Seung Ok Lee |
solee@chonbuk.ac.kr |
Gall Bladder & Biliary tree |
Background/Aims: Patterns of 18F-FDG PET in cholangiocarcinoma (CC) are variable and its usefulness has not been well established. To evaluate the usefulness and pattern of 18F-FDG uptake, CC patients were classified according to the tumor location and morphology. Methods: 82 patients (M:55, F:27, mean age 66.2) with CC were analyzed.Location of CC were 32%(n=26), 31%(n=25), 38%(n=31) in peripheral, hilar and distal, respectively. 56 patients of extrahepatic CC showed schirrhous in 61%(n=34), nodular in 16%(n=9), and polypoid in 23%(n=13) morphologically. PET positivity with peak SUV (pSUV) was evaluated. Results: Positive rates of 18F-FDG uptake were 88% in peripheral and 79% in extrahepatic (76% in hilar and 81% in distal). In PET positive cases, mean pSUV values were 9.4±4.1 in peripheral and 6.8±4.3 in extrahepatic (7.0±3.7 in hilar, 6.6±4.7 in distal). Mean pSUV of peripheral CC was significantly higher compared to extrahepatic (P=0.02). In extrahepatic CC according to morphology, positive rates of 18F-FDG uptake were 71%, 85%, 100% in schirrhous, nodular, andpolypoid, respectively. Mean pSUV were 5.5±2.9, 6.9±4.9, 10.1±7.0 in schirrhous, nodular, and polypoid, respectively. Mean pSUV of polypoid type was significantly higher compared to schirrhous type (P=0.01). Conclusions: Overall positive rate of 18F-FDG uptake on PET scan in CC was 82%. Peripheral type of CC showed higher positive rate with higher pSUV compared to extrahepatic CC. In extrahepatic CC, polypoid type showed higher positive rate with higher pSUV compared to schirrous type. These results suggest the difference of glucose metabolism in CC according to the tumor location and morphology. |
Dr. Soo Teik Lee |
Seung Ok Lee, Yoon Jae Lee, Seong Hun Kim, In Hee Kim, Sang Wook Kim, Dae Ghon Kim, Seok Tae Lim*, and Soo Teik Lee |
Department of Internal Medicine and Nuclear Medicine*, Chunbuk National University College of Medicine, Jeonju, Korea |
| PREDICTORS OF BONE MINERAL DENSITY (BMD) AT DIAGNOSIS AND OVER THE FIRST 12 MONTHS OF GLUTEN-FREE DIET (GFD) IN COELIAC DISEASE (CD) |
Ms. Sue J Shepherd |
sue@coeliac.com.au |
Nutrition |
CD is associated with reduced BMD and improvement with a GFD. Aim: To define clinical predictors of bone status at diagnosis and response of BMD over the first 12 months on a GFD. Methods: 99 patients (aged 18-71 years) consecutively referred for dietary management prospectively underwent DEXA to yield BMD and bone mineral content (BMC) at diagnosis. The first 57 underwent repeat DEXA after 12 months on a GFD (all strictly compliant). Results: At diagnosis, 35 had reduced BMD (T-score <-1) (27% osteopenia, 8% osteoporosis). No association was found with severity of symptoms, serological profile or severity of the villous lesion. After 12 months, only lumbar spine BMD improved overall (1.18±0.17 vs 1.21±0.16; p<0.001). Change in BMC correlated negatively with initial BMC (r=-0.30) and positively with weight (r=0.41) at diagnosis. In those with reduced BMC initially, BMC improved (p=0.001) by 2.5±0.04% compared with 0.7±0.03% in those who did not (p=0.3235). Taking calcium and/or vitamin D supplements (n=11) was associated with greater improvement (3.6±0.16% vs 0±0.11%; p=0.01). Gender and severity of the initial villous lesion or symptoms did not predict change in BMC. Multivariate analysis revealed only initial BMC and weight at diagnosis as independent predictors of change in BMC. Conclusions: Osteopenia/porosis at diagnosis cannot be predicted by standard clinical observations, but is the major predictor of improvement over the first 12 months’ GFD. All patients should be screened by DEXA at diagnosis but only those with initially reduced BMD require repeat scans. |
Prof. Peter Gibson |
Shepherd SJ, Strauss B, Hosking P, Gibson PR. |
Department of Gastroenterology, Box Hill Hospital & Body Composition Lab, Monash Medical Centre, Victoria, Australia |
| EFFECT OF PROTON PUMP INHIBITOR ON THE BONE TURNOVER IN THE OVARIECTOMIZED ICR MICE |
Dr. Beom Jae Lee |
hankoonhee@hanmail.net |
Miscellaneous |
INTRODUCTION: Taking PPIs for more than a year was associated with increased fracture risk by 44% while long-term, high-dose users had 2.6 times greater risk than nonusers, reported.AIMS & METHODS: The aims of this study were to evaluate the effect of PPI on the bone turnover in the ovariectomized ICR mice. 8 weeks-old ovariectomized female ICR mice were randomized into either PPI group or control group. PPI groups (n=10) and control groups (n=10) were injected with pantoprazole (40mg/kg/day, i,p) or normal saline (i,p) for 4 weeks respectively. Bone mineral density (BMD) at tibia, relative tibia weight and serum chemistries (ALP, Ca, Phosphorus) were measured. Serum levels of osteocalcin and CTX-1 were measured by ELIZA. The cytokines such as IL-1b, IL-6, and TNF-a were determined by RT-PCR. RESULTS: There were no differences in BMD, tibia relative weight and serum chemistries between two groups(p>0.05). Serum levels of osteocalcin in pantoprazole groups were significantly lower than that of control groups (p=0.023), but no difference found at the serum levels of CTX-1(p>0.05).The expression of IL-1b in spleen was suppressed significantly in pantoprazole group, but there were no significant differences at the expressions of TNF-a and IL-6 between two groups. CONCLUSION: The pantoprazole did not affect the bone mineral density, serum chemistry and osteocalcinogenesis in the ovariectomized ICR mice. However, the pantoprazole might have a possibility to effect on the bone turnover by the suppression of osteoblastogenesis |
Prof. Jong-Jae Park |
Beom Jae Lee, Jong-Jae Park, Moon Kyung Joo, Sung Joo Chung, Chung Ho Kim, Seung Kwon Hong, Jong Hwan Choi, Young Gul Jung, Ji Hoon Kim, Jae Seon Kim,Jong Eun Yeon,Sang Woo Lee, Kwan Soo Byun,Young-Tae Bak |
Department of Internal Medicine, Korea University Guro Hospital, Seoul, South Korea |
| Eupatilin dissociates the Hsp90 and IKK-gamma complex leading to suppression of NF-kB-regulated gene expression in intestinal epithelial cells stimulated with Bacteroides fragilis enterotoxin |
Prof. Jung Mogg Kim |
jungmogg@hanyang.ac.kr |
Large Intestine |
Eupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone), a pharmacologically active flavone derived from Artemisia asiatica, protects against intestinal injury and inflammation. In this study, we characterized the anti-inflammatory molecular mechanisms of eupatilin in intestinal epithelial cells stimulated with toxin produced by enterotoxigenic Bacteroides fragilis (BFT). Pretreatment of HT-29 intestinal epithelial cells with eupatilin significantly decreased the production of both IL-8 and prostaglandin E2 (PGE2) induced by BFT in a dose-dependent manner. BFT activated NF-kB signals in HT-29 cells and pretreatment with eupatilin suppressed NF-kB activation that resulted in the significant inhibition of IL-8 and cyclooxygenase-2 (COX-2) expression. BFT-induced IkB-alpha degradation was prevented in eupatilin-pretreated cells, and eupatilin significantly reduced the BFT-stimulated enhanced activity of both IkB kinase (IKK) and phospho-IkB-alpha. Transfection of siRNA for IKK-alpha and IKK-beta decreased the production of IL-8 and PGE2, however, transfection of IKK-beta siRNA showed more significant reduction of the BFT-induced IkBa phosphorylation compared with that of IKK-alpha siRNA. In addition, a specific inhibitor of heat shock protein 90 (Hsp90), herbimycin A, decreased the activities of IKK and NF-kB upregulated by BFT stimulation, suggesting that Hsp90 is associated with expression of IKK-NF-kB-IL-8/COX-2 gene. Furthermore, eupatilin dissociated the complex between Hsp90 and IKK-gamma in BFT-stimulated HT-29 cells. These results indicate that eupatilin suppresses the NF-kB signaling pathway by targeting the IKK complex in intestinal epithelial cells and attenuates the BFT-induced inflammatory response, suggesting that eupatilin may be a possible therapeutic strategy for treatment of inflammation caused by toxigenic B. fragilis infection. |
Prof. Jung Mogg Kim |
Jung Mogg Kim1, Joo Sung Kim2, Dong Ho Lee2 |
1 Department of Microbiology, Hanyang University College of Medicine; 2 Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea |
| The use of Rockall Score in predicting mortality, recurrent bleeding and need for surgery in non-variceal upper gastrointestinal bleeding at a University Hospital in the East Coast of Peninsular Malaysia |
Dr. Yeong Yeh Lee |
justnleeyy@gmail.com |
Stomach (Non H – Pylori) |
Introduction Non-variceal upper gastrointestinal bleeding (NVUGIB) is common in the East coast of Peninsular Malaysia. There was no published study yet on the use of Rockall score in NVUGIB from this area. Methods 250 patients with NVUGIB who were admitted to Hospital University Sains Malaysia (HUSM) were enrolled between 2004 and 2006. Results The mean age was 62.1 +16 years. There were more males (n=144 or 57.6%) and Malays (n=209 or 83.6%). The mortality rate was 3.6% (9 patients), recurrent bleeding rate was 9.6% (24 patients) and rate requiring surgery was 4.4%(11 patients). Most patients (85.2% or n=213) were in the low risk group (Rockall score ¡Ü 5). The high risk group (Rockall score>5) was significantly associated with mortality (p < 0.001), recurrent bleeding (p value = 0.01) and need for surgery (p = 0.013). Factor associated with mortality in multivariate analysis was sepsis (p=0.021, OR=9.9, 95% CI 1.413-69.513). Factor associated with recurrent bleeding in multivariate analysis was a higher creatinine level (p=0.012, 95% CI 1.0-1.0). Factors associated with the high risk group in multivariate analysis were stigmata of recent haemorrhage (p<0.001, OR=0.063, 95%CI =0.26-0.152), sepsis (p=0.013, OR=0.149, CI 0.034-0.664) and warfarin use (p=0.028, OR=0.182, CI = 0.040-0.832). Conclusion High risk group (Rockall score>5) was associated with a higher mortality, recurrent bleeding and need for surgery but not low risk group. Sepsis was found to be an important risk factor for a higher mortality in NVUGIB. |
Dr. Yeong Yeh Lee |
1. Lee Yeong Yeh 2. Noridah Nordin 3. Amry A Rahim 4. NorAizal Che Hamzah 5. Nazri Mustaffa 6. Maya Mazuwin 7. Zaidi Zakaria 8. Syed Hassan Syed Aziz |
1-5 Department of Medicine 6-8 Department of Surgery University Sains Malaysia, 16150 Kubang Kerian, Kota Bahru, Kelantan, Malaysia |
| Mucosal Mastocytosis as a Histological Marker in Diarrhea Predominant Irritable Bowel Syndrome |
Mr. P Patrick Basu |
patbasumd@aol.com |
Miscellaneous |
Purpose: Irritable Bowel Syndrome (IBS) affects approximately 1 in 5 (20%) adults in USA, particularly women. An exact etiology of IBS is controversial; however it is a functional disarray of the brain-gut axis, changes in the intestinal flora and gut immune dysregulation. ROME III criteria are the gold standard. No specific serological, immunological or histological markers exist. We propose rectal mucosal mast cell aggregation in the pathogenesis of IBS. Methods: 120 patients (72 women, 48 men, age range-20 to 50 years, met ROME III criteria) with diarrhea predominance (>3 bowel movements (BM) per day, n=78), pure constipation (<3 BM per week, n=8) and mixed symptoms (n=34) were included. Detailed clinical history including onset, pattern and symptom duration was taken. Laboratory tests included blood counts, Basic Metabolic Panel, thyroid function, Inflammatory Bowel Disease (IBD) and celiac panels, stool analysis for C. difficile toxin, ova and parasites. Patients with blood in stool, weight loss, celiac disease, systemic mastocytosis and IBD were excluded. All patients had flexible sigmoidoscopy and rectal biopsies. (Caris labs, Irvin,TX) Results: Despite a normal macroscopic appearance, 109/120 (91%) {diarrhea predominant 78/78 (100%), pure constipation 7/8 (87.5%) and mixed symptoms 24/34 (70.5%)} had >20 mast cells per high power field (hpf) with Tryptase immunostain. Conclusions: We propose a unique histological entity, mast cell proctopathy (MCP), that appears to be a specific histological marker for diarrhea predominant IBS and may play a role in the immuno-pathogenesis. A large prospective trial with random biopsies throughout the colon will further establish this feature. |
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| Stretta precedure for the Treatment of GERD mainly with extra-esophageal symptoms: one year follow up |
Mr. Jimin Wu | drwujimin@yahoo.com.cn |
Esophagus |
Purpose: To explore the effectiveness of Stretta radiofrequency (RF) to treat gastroesophageal reflux disease (GERD) with mainly extra-esophageal symptoms. Methods: From April 2006 to February 2007, 168 consecutive GERD patients with mainly extra-esophageal presentations were treated by the RF. Among them, 148 (88.1%) patients had asthma, 118(70.2%)patients had cough, 72(42.9%)patients had pharyngolaryngitis, 142(84.5%)patients accompanied with regurgitation or heartburn. Follow up were made 1, 3, 6, and 12 months respectively after RF treatment. Results: 164 patients were treated under conscious sedation and four were under general anesthesia,the average operating time was 39.0min. Minor complications included temporary post-procedural retrosternal unease or pain, mild fever, transient nausea/vomiting, and transient dysphagia. There were no post-procedure perforations, mucosal lacerations, or deaths,and there were no late complications such as stricture formation, new dysphagia, or achalasia. A RF redo was performed in 2 patients because of recurrence. After RF, 160 (95.2%) patients obtained follow-up, 51(31.9%) patients had complete resolution of their symptoms, 69 (41.1%) patients had symptoms remarkably relieved, 33 (20.6%) had partly relieved, 7 (4.4%) patients had no effect. According to our score system, the symptom score fell from 4.17 to 1.44 in average one year after the procedure. Conclusions: The Stretta RF is safe and effective in the control of extra-esophageal symptoms in patients with gastro-oesophageal reflux disease. Key Words: gastroesophageal reflux disease; asthma; extra-esophageal symptom:Stretta radiofrequency: gastroscopy |
Dr. Wang Zhonggao |
Wu Jimin1; Wang Zhonggao 1£¬2, Liu Jianjun1, Wang Liyin1, Tian Shurui 1 |
1 Center for GERD, The Second Artillery General Hospital, Clinical Research College of Beijing Normal University, Beijing; 2 Department of Thoracic Cardiovascular Surgery, Xuan Wu Hospital, Capital University of Medical Science |
| Nutrition In Severe Acute Pancreatitis (SAP) |
Prof. Rakesh Kochhar |
dr_kochhar@hotmail.com |
Pancreas |
Background: We compared the effect of total enteral nutrition (TEN) and total parenteral nutrition (TPN) in the management of SAP. Methods: Fifty consecutive patients with SAP (CTSI>7) were prospectively randomized to receive TEN (n=25, age 38.4±13.8yrs) or TPN (n=25, age 41.08±11.34yrs). Serum CRP, albumin, transferrin levels, organ failure, surgical intervention, duration of hospital stay and mortality were evaluated. Results: Following 14 days of TEN/TPN, serum CRP decreased significantly in both TEN (5.6mg/dl from 102.4 mg/dl; p-0.0) and TPN groups (3.20mg/dl from 102.4mg/d; p=0.0); serum albumin increased significantly in TEN (2.82±0.51 to 3.34±0.45 g/dl; p- 0.00) but not in TPN group (3.1±0.59 to 3.21±0.3g/dl; p-0.64). A significant rise in serum transferrin was observed in both TEN (p-0.0) and TPN group (p-0.04). Increase in CTSI score was significant in TPN (9.26 from 8.7; p-0.02) but not in TEN group (9.08 from 8.84; p-0.10). There was no significant difference in surgical intervention (53.8% Vs 57.7% p=0.774), infective complications (64% vs 60%; p-0.66), mean hospital stay (42.68±21.7 Vs 42.68±17.3days), or mortality (19.2% Vs 15.4%; p-0.6) in TEN and TPN groups. E. coli (54.5%) and Staph. aureus (30.56%) were the commonest organisms isolated among the TEN and TPN patients respectively. Conclusion: Both TEN and TPN were equally effective in terms of fall in CRP levels, rise in transferrin levels, need for surgery, infective complications, hospital stay and mortality. TEN was however more effective in increasing serum albumin levels and was not associated with an increase in CTSI. |
Dr. Rakesh Kochhar |
Dole RP, Poornachandra KS,Yadav TD, Gupta R, Gupta V, Kochhar R, Vaishnavi C, Singh K, Wig JD |
Department of Gastroenterology and GI Surgery, Postgrad Institute of Medical Education and Research, Chandigarh |
| Hepatitis E viral quantification in patients of Fulminant Hepatic Failure by Real Time PCR |
Mrs. Nargis Begum |
nargisbegum18@gmail.com |
Liver – Viral Hepatitis |
Hepatitis E virus (HEV) infection is known to cause severe liver disease in pregnant women. The duration of viremia HEV infection has rarely been reported. The present study was undertaken to investigate the viral load of HEV viremia in serum samples collected from pregnant and non-pregnant subjects with fulminant hepatic failure. HEV RNA was quantitated in periodic serum specimens obtained from 10 subjects (5 pregnant and 5 non-pregnant) on following days (0,7,15,30,60,90) with sporadic acute hepatitis E. All 10 patients had detectable HEV RNA in serum at admission, with the highest viral load being 1.1 × 106 copies/ml in FHF pregnant and 1.2 x 105 copies/ml in FHF non-pregnant subjects. The HEV viral load declined as the days of follow up increased. HEV viremia had lasted for 30 days in 3 pregnant subjects with an average viral load of 3.1 x 104 copies/ml and in 1 non-pregnant subject with viral load of 1.4 x 104 copies/ml after the onset of hepatitis. There was a relatively higher viral load in FHF pregnant subjects than non-pregnant. The proportion of HEV viremia and its duration were not directly related to serum ALT values. It seems to be related to the disease severity (i.e. in the period of encephalopathy). |
Dr. Premashis Kar |
Nargis Begum1,2,3, Mohd. Asim1,2, Sunil K Polipalli1, Ashok Kumar3, SA Husain2, P Kar1 |
1. PCR Hepatitis Lab, Dept. of Medicine, Maulana Azad Medical College, New Delhi-110002. 2. Dept. of Biosciences, Jamia Millia Islamia, New Delhi-110025. 3. Dept. of Obsts. & Gynae, Maulana Azad Medical College, New Delhi-110002 |
| Endoscopic Balloon Dilatation – An Effective Alternative To Surgery In Caustic Induced Gastric Outlet Obstruction |
Prof. Rakesh Kochhar |
dr_kochhar@hotmail.com |
Endoscopy |
OBJECTIVE : To study the response of Endoscopic Balloon Dilatation (EBD) in patients with caustic induced Gastric Outlet Obstruction (GOO). METHOD : Patients with caustic GOO (stricture segment <2.5 cm) treated in the last 10 years were analysed for the number of dilatations and outcome. EBD was done using TTS balloon, in 27 patients presenting within 8 weeks (Group I, 17 males, age 35.5±12.5 yrs) and in 41 patients presenting after 8 weeks (Group II, 23 males, age 29.6±8.5 years) of caustic ingestion. Success was defined as achieving gastric dilatation of 15 mm and being symptom free thereafter. Patients were then followed up for recurrence. RESULTS : In group I, 26(96%) patients underwent successful dilatation, requiring 9.26±3.4 sessions at weekly interval to achieve the end point. Mean size of first dilator was 9.04±1.28 (range 8-12 mm). One patient developed large prepyloric ulcer and was subjected to surgery. In group II, 39(95%) patients underwent successful dilatation requiring 5.83 ±2.58 (2-13) sessions at three weekly interval to achieve the end point. Mean size of first dilator was 8.2±0.6 mm (range 8-10 mm). One patient in group II had perforation, another had pain during EBD; both were operated upon. Group I patients were followed up for 13.19±12.10 months and group II for 35.4±11.1 months without recurrence. CONCLUSIONS : Selected patients with GOO in both subacute and chronic phase can be successfully dilated using EBD with 95% success rate. Thus EBD is an effective alternative to surgery in such patients. |
Prof. Rakesh Kochhar |
Agrawal A, Jain K, Dutta U, Nagi B, Kochhar R, Kochhar S, Singh K. |
Departments of Gastroenterology and Radiodiagnosis, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh – 160012. |
| An association of N34S SPINK1 mutation with alcoholic pancratitis in north India |
Ms. Divya Singh | divya_guriya@rediffmail.com |
Pancreas |
Background and aim- The mechanism by which alcohol causes pancreatitis is not known. Many who drink large amounts may not develop the disease, while several may manifest with consumption of modest doses suggesting of genetic susceptibility. The N34S SPINK1 gene mutation is associated with tropical chronic pancreatitis (TCP). Whether this mutation predisposes subjects consuming alcohol to pancreatitis is not well known. Thus our aim was to investigate the prevalence of N34S SPINK1 mutation in alcoholic pancreatitis (AP) patients and TCP in comparison with healthy control (HC). Method- Blood samples were obtained from patients with TCP (n=51), AP (n=40) & healthy controls (n=40). DNA extraction was done by phenol-chloroform method and N34S SPINK1 mutation was detected by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). Genotyping was done on 3% agarose gel. Result- The frequency of N34S SPINK1 mutation in TCP was 47 % (24/51), in AP 32.5 % (13/40) and 2.5 % (1/ 40) of healthy controls. This mutation was significantly higher in AP than healthy controls (p=0.003). N34S SPINK1 mutation was also more frequent in TCP group in comparison to HC (p=0.001), the frequency being similar what we had observed earlier. Statistically similar frequency was observed in TCP and AP patients. Conclusion- We observed that N34S SPINK1 mutation which had been earlier reported to be associated with TCP was significantly increased in our patients of AP as well. This suggests that mutation in this disease modifier gene could predispose to injury by environmental toxins such as alcohol. |
Dr. Gourdas Choudhuri |
Divya Singh, TS Negi, Eesh Bhatia*, Gourdas Choudhuri |
Department of Gastroenterology, Department of Endocrinology*, SGPGIMS, Lucknow. |
| Abstract Title |
Abstract Author |
Email |
| ENDOSCOPY | |
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| Healing of solitary rectal ulcer with multiple sessions of argon plasma coagulation. |
SANJAY KUMAR SOMANI | sksomani@ymail.com |
| Appropriateness of referrals for Esophagogastroduodenoscopy (EGD) and its association with endoscopic findings |
Bickram Pradhan | bickram_11@hotmail.com |
| Postpolypectomy Bleeding In Patients Undergoing Colonoscopy With Uninterrupted Clopidogrel Use: Time To Revisit The Guidelines |
VIVEK KAUL | VIVEK_KAUL@URMC.ROCHESTER.EDU |
| Does routine upper GI endoscopy help in pre-renal transplant workup? |
Abhai Verma | drabhaiv@yahoo.com |
| Gastrointestinal Polyposis and Cutaneous Changes- Cronkhite Canada Syndrome |
Amitabh Monga | Amitabh_Monga@ttsh.com.sg |
| Small sphincterotomy combined with papillary dilation with large balloon permits retrieval of large biliary stones without mechanical lithotripsy |
Atsushi Minami | minamia@d1.dion.ne.jp |
| Inhaled methoxyflurane as a patient controlled analgesic agent for colonoscopy |
BIJU GEORGE | drbijugeorge@gmail.com |
| Inverted diverticulum simulationg colon polyp. |
Daejin Kim | cognex@dreamwiz.com |
| Renal cell carcinoma presenting as antro-duodenal obstruction |
Debabrata Banerjee | drdbanerjee@gmail.com |
| Endoscopic management of pancreatic pseudocysts at atypical locations |
Deepak Bhasin | deepakkbhasin@gmail.com |
| Outcome of Savary Gilliard Dilatation of Esophageal anastomotic strictures secondary to surgical repair of Esophageal Atresia in infants and young children in tetiary hospital |
mohanreddy gongala | drmohangreddy@gmail.com |
| Assessment of Patient Comfort Levels During Endoscopic Procedures |
Ravi Madhotra | ravimadhotra@yahoo.com |
| A case of advanced gastric cancer resembling submucosal tumor of the stomach |
Dizza Dujali | deric_dy@yahoo.com |
| Upper GI endoscopy is safe and useful in orophagryngeal dysphagia |
khawaja Qamaruddin Sediqi | drsediqi007@hotmail.com |
| Palliative management of irresectable carcinoma esophagus using SEMS |
Muhammad Fahad Tariq Berlas | drfahadtariq@yahoo.com |
| A prospective randomized comparison of oral polyethylene glycol and sodium phosphate lavage for colonoscopy preparation |
Peng LI | zhangst@ccmu.edu.cn |
| Papillary Deformities associated with Chronic Duodenal Ulcer Disease |
Vivekanand Kulkarni | vkantrang@yahoo.co.in |
| Endoscopic Sclerotherapy Vs Endoscopic Variceal Ligation in the management of Variceal Bleeding |
Sunil Kumar Jangala | drjsunil@yahoo.com |
| Pancreatic ascites: Study of 25 patients with endoscopic intervention |
Harshad Devarbhavi | harshad.devarbhvavi@gmail.com |
| EUS findings and colonoscopic treatment of two cases of granular cell tumor of the colon |
Jeong Eun Shin | dreun1028@yahoo.co.kr |
| Colorectal Neoplasm in Symptomatic Indonesian Population: Result of Colonoscopy Examination |
Julwan Pribadi | murdani@cbn.net.id |
| The early colonoscopy following computed tomography for diagnosing appendicitis or diverticulitis in the patients with acute RLQ pain |
kwang An kwon | toptom@gilhospital.com |
| Complications of endoscopy, an audit and analysis of outcomes from a tertiary care centre |
Lakshmi CP | dr_lakshmi_cp@yahoo.co.in |
| Endoscopic submucosal dissection as a new therapeutic modalities for rectal carcinoid |
Dr. Min Kyu Jung | minky1973@kornet.net |
| The Effect of Oral Cola Ingestion for the Endoscopic Inspection of Remnant Stomach - Randomized Case Control Study - |
Moon Kyung Joo | gi7pjj@yahoo.co.kr |
| Clinical and endoscopic features of gastric varices secondary to splenic vein occlusion |
Takahiro Sato | taka.sato@ja-hokkaidoukouseiren.or.jp |
| Cap Assisted Gastroscopy : A Review of A Case Series |
Raymond Sebastian | bastian_10@yahoo.com |
| “STRAIGHT – TO – TEST” UPPER GI CANCER SERVICE: EFFICIENCY, SAFETY AND COST EFFECTIVENESS |
Ravi Madhotra | ravimadhotra@yahoo.com |
| Alarm Symptoms and Upper Gastrointestinal Malignancy in Primary Care Endoscopy. |
Mohammed Masudur Rahman | drmasud47@yahoo.com |
| Deciding the end point of palliative endoscopic biliary drainage and study of efficacy and outcome in patients with malignant biliary obstruction |
Rakesh Kumar | call_rakesh@rediffmail.com |
| Real Time Confocal Endomicroscopy Accurately Diagnoses Celiac Disease at the Time of Endoscopy. |
Rupert Leong | rupertleong@hotmail.com |
| Non surgical management of pancreatic fluid collections |
Nagaraj Kotli | drnagarajkotli@gmail.com |
| Major Indications For Upper Gastrointestinal Endoscopy And Their Outcome In Adult Sri Lankan Population – Experience In A Medical Unit Of A Suburban Secondary Referral Center |
Ravindra Satarasinghe | ravindrasatharasinghe@yahoo.com |
| Comparison Of Clinical Features And Demographics Of Sri Lankan Alcoholics And Non Alcoholics Who Had Primary Prophylactic Banding Ligation Of Significant Esophageal Varices (Grade 3-4) Admitted To A Medical Unit Of A Tertiary Care Hospital |
Ravindra Satarasinghe | marjayawardana@yahoo.com |
| Factors affecting Bile Duct clearance at Endoscopic Retrograde Cholangiography in patients with Choledocholithisis. |
Nisha Kapoor | akseth2003@yahoo.com |
| General Anaesthesia or Conscious Sedation for Endoscopic Retrograde Cholangio-Pancreatography: Does it make a difference? |
Prof. Avnish Kumar Seth | akseth2003@yahoo.com |
| Antral retch gastropathy: A new cause of upper gastrointestinal bleeding |
SP Misra | drspmisra@gmail.com |
| A study of grading system to evaluate small bowel cleansing for wireless capsule endoscopy |
Hoon Jai Chun | drchunhj@hanmail.net |
| Analysis for Factors Affecting on Procedure Time during Endoscopic Submucosal Dissection |
Sung Jae Shin | jinhkim@ajou.ac.kr |
| COMPARISON OF UNCOVERED AND COVERED STENT FOR ENDOSCOPIC TREATMENT OF INOPERABLE MALIGNANT GASTRODUODENAL OBSTRUCTION |
Sung Jae Shin | jinhkim@ajou.ac.kr |
| A Comparative Study Between MRCP (Magnetic Resonance Cholangiopancreato graphy) and ERCP (Endoscopic Retrograde Cholangiopancreato graphy) In Pancreatobiliary Diseases |
SURENDAR REDDY BARADHI | surendarbaradhi@yahoo.co.in |
| Diagnosis of esophageal and gastric carcinoma using transnasal ultrathin esophagogastroduodenoscopy |
Takashi Kawai | t-kawai@tokyo-med.ac.jp |
| Impact of transnasal ultrathin esophagogastroduodenoscopy (UT-EGD) in the evaluation of esophageal peristaltic function |
Takashi Kawai | t-kawai@tokyo-med.ac.jp |
| Analysis of correlation study between endoscopic findings and clinical features in the patients with gastric anisakiasis in Japan. |
Tatsuya Hoshi | yuki_hiro8260@yahoo.co.jp |
| Patients’ preference for gender of endoscopists and assistants during colonoscopy: Implication for screening colonoscopy |
Anand JALIHAL | chongvuih@yahoo.co.uk |
| A Comparison of Metal and Plastic Stents for the Relief of Jaundice in Unresectable Malignant Biliary Obstruction in Korea-An Emphasis on Cost-effectiveness |
Won Jae Yoon | reich@hanafos.com |
| Role of combination of the mini-probe EUS and the EUS in the preoperative staging for colorectal carcinoma |
Dr. Lu Xia | xialu@medmail.com.cn |
| LIVER: VIRAL HEPATITIS |
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| Serum AST Is A Better Predictor Of Necro-Inflammation Than Serum ALT In Patients With Wild Type CHB: Experience From A Tertiary Centre In Bangladesh |
Mamun Mahtab | hbd@dhaka.net |
| Serum ALT Is A Better Predictor Of Necro-Inflammation Than Serum AST In Patients With Pre-Core Mutant Type CHB: Experience From A Tertiary Centre In Bangladesh |
Mamun Mahtab | hbd@dhaka.net |
| HBeAg Positive CHB Is Associated With Higher HBV DNA Than HBeAg Negative CHB |
Mamun Mahtab | hbd@dhaka.net |
| HBeAg Positive HBV Produces More Severe Liver Disease Than HBeAg Negative CHB |
Mamun Mahtab | hbd@dhaka.net |
| Genotypes Of HCV In Bangladesh: Experience From A Tertiary Centre |
Mamun Mahtab | hbd@dhaka.net |
| Prevalence Of HBeAg Negative HBV Infection In Bangladeshi IDHAS |
Mamun Mahtab | hbd@dhaka.net |
| Vertical transmission of Hepatitis B virus is the major route of transmission in asymptomatic carriers in Bangladesh |
Harun-or Rashid | drharun_bd@yahoo.com |
| Steatosis, Insulin resistance, Iron overload, Fibrosis and Viral load as negative factors affecting early (EVR) and sustained (SVR) Virological Response in patients with Chronic Hepatitis C treated with peginterferon and ribavirin |
Eugen Georgescu | efg@usa.net |
| Elevated Toll-like receptor 2 on the hepatocytes in patients with chronic hepatitis B: Relationship With the Histological Grading. |
Wei Xiuqing | weixiuqing1975@yahoo.com.cn |
| Elevated Toll-like receptor 4 on the hepatocytes in patients with chronic hepatitis B: Relationship With the Histological Grading. |
Wei Xiuqing | weixiuqing1975@yahoo.com.cn |
| Elevated expression of toll-like receptors 2 in hepatocellular carcinoma. |
Wei Xiuqing | weixiuqing1975@yahoo.com.cn |
| Entecavir at Five Years Shows Long-Term Maintenance of High Genetic Barrier to Hepatitis B Virus Resistance |
BL Neo | genevieve.bulluss@meditechmedia.com |
| Long-term Follow-Up of Entecavir Treated Protocol-Defined Non-Responders in Rollover Study ETV-901 |
Jose Sollano | genevieve.bulluss@meditechmedia.com |
| Long Term Efficacy, Safety and Resistance Analyses of Entecavir (ETV) Treatment in Japanese Nucleoside-Naive Patients with Chronic Hepatitis B (CHB) |
Masao Omata | genevieve.bulluss@meditechmedia.com |
| BIOCHEMICAL, HISTOLOGIC AND VIRAL FACTORS INFLUENCING EARLY (EVR) AND SUSTAINED (SVR) VIROLOGICAL RESPONSE IN PATIENTS WITH CHRONIC HEPATITIS C TREATED WITH PEGINTERFERON PLUS RIBAVIRIN |
Eugen Georgescu | efgmed@gmail.com |
| Severity of Acute Hepatitis and Outcome in Patients with Dengue fever at a Tertiary care center. |
Om parkash | drom73@yahoo.com |
| Efficacy of Entecavir in Hepatitis B Virus (HBV) related chronic liver disease patients in a Tertiary Care from South India – An open label study data |
Rajesh Gupta | npadaki@yahoo.com |
| Attack Rate of Hepatitis E Virus (HEV) Infection in Children during Epidemic Hepatitis E |
Mehnaaz Khuroo | khuroo@drkhuroo.org |
| ROLE OF ORAL ANTIVIRAL DRUGS IN TRANSFUSION-ASSOCIATED HEPATITIS B INDUCED LATE ONSET HEPATIC FAILURE |
AJAY KUMAR JAIN | ajayvjain@yahoo.com |
| Hepatitis B Vaccination In Non HBV Chronic Liver Disease |
Nayana Joshi | nay_joshi@yahoo.com |
| HBV Genotypes In India: Do They Influence Disease Severity? |
Kaushal Madan | subratacharya2004@yahoo.com |
| Study of regulatory polymorphism of TNF ligand and receptor superfamily, member 6 in HCV related liver diseases |
Shyam Swargiary | swargiary.ss77@gmail.com |
| Phylogenetic analysis of HEV isolates in patients with HEV related acute liver failure from Rajasthan |
Nidhi Singh | rameshroop@gmail.com |
| Spectrum of Hepatitis Infection, Viral-Host Factor interaction associated with the Severity of Liver Disease in Assam-A Pilot Study |
bharti baruah | sujoybose1@gmail.com |
| Entecavir in asymptomatic HBV carriers |
Ajit Kumar | ajitkrs@yahoo.com |
| Efficacy of Entecavir in Lamivudine resistant Hepatitis B patients |
P Piramanayagam | gourdas@satyam.net.in |
| Serological study of hepatitis A virus infection amongst the students of a medical college in Cuttack and evaluation of the need of vaccination |
Shivaram Prasad Singh | scb_gastro_dept@hotmail.com |
| Association of High viral load and hepatitis B virus genotype D increases the risk of hepatocellular carcinoma. |
Asim Mohammad | pkar@vsnl.com |
| Screening Precore Mutation G1896A in Patients of Chronic Liver Disease by Ligase Chain Reaction |
Premashis Kar | premashishkar@gmail.com |
| Correlation of hepatocyte expression of hepatitis B viral antigens with histological activity and viral titer in chronic hepatitis B virus infection – an immunohistochemical study |
Banumathi Ramakrishna | banu@cmcvellore.ac.in |
| A study of clinical features of acute hepatitis A in recent 2 years in Korea |
So Young Kwon | sykwonmd@hotmail.com |
| Evaluation of Hepatitis B Viral markers in patients with chronic liver disease in Orissa |
Shivaram Prasad Singh | scb_gastro_dept@hotmail.com |
| Treatment of Chronic Hepatitis C with Pegylated / Conventional Interferon Alpha2b and Ribavirin: Factors determining response to therapy in India. |
Sanjeev Rastogi | akseth2003@yahoo.com |
| Fulminant hepatic failure in pregnancy: aetiology and predictors of outcome. |
Bilal-ul Rehman | showkatzargar@yahoo.com |
| Defective Notch Signaling; Attenuted Expression of Notch1, Notch4 and HES1 in Hepatitis B infection |
Nirupma TrehanPati | sukritibiochem@gmail.com |
| Association of Insulin Resistance and Steatosis to Host and Viral Factors in Chronic Hepatitis C |
Omesh Goyal | goyalomesh@yahoo.co.in |
| Liver function derangement and bleeding morbidity in Dengue Hemorrhagic Fever (DHF) |
Yeong Yeh Lee | justnleeyy@gmail.com |
| Dynamics of HEV viremia, fecal shedding and its relationship with transaminases and antibody response in sequential samples from patients with sporadic acute hepatitis E |
Harsh Udawat | rameshroop@gmail.com |
| Correlation of MELD with King’s College Hospital Criteria in Non Alcohol and non acetaminophen induced Acute Liver Failure |
om Parkash Devpal | om.parkash@aku.edu, drom73@yahoo.com |
| CLINICAL AND MOLECULAR EPIDEMIOLOGY OF HEPATITIS C VIRUS IN NORTH-EASTERN INDIA: OCCURRENCE OF 4A AS THE MAJOR SUBTYPE. |
Subhash Medhi | medhi_79@yahoo.co.uk |
| Neuropsychological impairment in severe acute viral hepatitis is due to minimal hepatic encephalopathy. |
Praveen Sharma | drpraveen_sharma@yahoo.com |
| Evolving a multiple assay system to detect Hepatitis B virus DNA, Hepatitis C and Hepatitis E virus RNA by simultaneous in patients with liver disease who hav coinfection or superinfection |
Gunjan Garg | drnishy7@gmail.com |
| In silico ANALYSIS OF PROMOTER REGION POLYMORPHISM AND EXPRESSION PROFILE OF TLR3 IN CHRONIC HEPATITIS C VIRUS (HCV) INFECTION |
Subhash Medhi | medhi_79@yahoo.co.uk |
| Rapid and early virological response as predictors of sustained virological response in chronic HCV infection |
piramanayagam P | piraman2000@yahooco.in |
| Natural history of hepatitis C virus infection in Indian Patients |
Nikhil Patel | niyopatel@yahoo.com |
| Vertical transmission of Hepatitis B virus is the important route of transmission in asymptomatic carriers in Bangladesh |
Harun-or Rashid | drharun_bd@yahoo.com |
| Role of Nuclear Transcription Factor and Antigen Processing , Presentation Machinery In Persistence Of Infection In Viral Hepatitis. |
Sukriti Sukriti | sukritibiochem@gmail.com |
| Hepatitis E virus is the major cause of Hepatic decompensation in patients with NAFLD in Bangladesh |
Harun-or Rashid | drharun_bd@yahoo.com |
| Predictors of thyroid dysfunction in patients with chronic hepatitis C during peginterferon and ribavirin combination therapy |
Eun Sun Jang | jeses19@gmail.com |
| Poor Pregnancy Outcome in Hepatitis E and Association of PROGINS: A Haplotype of Progesterone Receptor |
Purabi Deka | deka.purabi@gmail.com |
| High Prevalence of Feco-Orally Transmitted Hepatitis A Virus from North-East India: A Pilot Study. |
Rajib Hazam | hazam3265@gmail.com |
| A comparative evaluation of Adefovir and Lamivudine in patients of Chronic Hepatitis B , correlation with HBV viral kinetics, hepatic necro-inflammation and fibrosis |
Pradeep S | grayneo@yahoo.com |
| Retreatment of HCV relapsers and non responders on interferon treatment |
Piramanayagam P | piraman2000@yahooco.in |
| Seroprevalence and Risk Factors Associated With Hepatitis B Virus Infection: A Population Based Study In Slum People of Dhaka City, Bangladesh. |
ABM Safiullah | safigastro@yahoo.com |
| Prevalence of Hepatitis D in HbsAg positive patients visiting the Liver clinics in Pakistan. |
Naresh seetlani | drnaresh2004@yahoo.com |
| The Clinical Significance of Interferon Inducible Protein-10 in Chronic Hepatitis C Patients With Genotype I Undergoing Pegylated Interferon and Ribavirin Therapy |
Mr. CHUNGHO KIM | Mr. CHUNGHO KIM |
| LIVER OTHERS | |
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| Amoebic liver abscess: A prospective study of parameters predicting biliary communication after percutaneous catheter drainage |
Uday Ghoshal | udayghoshal@yahoo.co.in |
| The Spectrum Of Histologically Diagnosed Major Hepatic Disorders In A Cohort Of Adult Sri Lankans – Experience In A Tertiary Care Hospital. |
Ravindra Satarasinghe | marjayawardana@yahoo.com |
| Comparison Of The Clinical Outcome Of Intravenous LOLA Administration For Hepatic Encephalopathy And Other Related Matters In Two Cohorts Of Patients Having Advanced Liver Disease Due To Alcoholic And Non Alcoholic Aetiology – A Pilot Study |
Ravindra Satarasinghe | marjayawardana@yahoo.com |
| THE EFFECTS OF SOME HERBAL MEDICINES ON BLOOD COAGULATION OF RATS WITH LIVER CIRRHOSIS |
Badamsuren Dorjgotov | tserendagva@hsum.edu.mn |
| A case of hepatic fascioliasis presenting as pyogenic liver abscess |
Daejin Kim | cognex@dreamwiz.com |
| A case of hepatic fascioliasis presenting as pyogenic liver abscess. |
Daejin Kim | cognex@dreamwiz.com |
| Repeat Trans-arterial Chemoembolization for Unresectable Hepatocellular Carcinoma: Efficacy and Long Term Survival |
Devinder Bansi | osama.damrah@imperial.ac.uk |
| Alcohol and HCV chronic infection are risk cofactors of type 2 Diabetes Mellitus for Hepatocellular Carcinoma in Italy |
Valter Donadon | valter.donadon@aopn.fvg.it |
| Spectrum of alcoholic liver disease, disparity between smokers and non-smokers |
Ramasubramanian no | poornima_deen@yahoo.com |
| Tuberculosis of the liver: twenty one years experience from a medical center in eastern Taiwan |
Lu-Chin Huang | luchin_huang@tzuchi.com.tw |
| Treatment of Liver Metastasis from Gastrointestinal Stromal Tumours: Multimodal Approach |
Devinder Bansi | osama.damrah@imperial.ac.uk |
| Discovery of Serum Biomarkers for Liver Fibrosis Detection by Two-Dimension Difference Gel Electrophoresis (2D-DIGE) |
Ho Ai-Sheng | aisheng49@yahoo.com.tw |
| Lympedim® (A Coumarin Derivative) Induced Reversible Hepatotoxicity In An Adult Sri Lankan - A Case Report from Sri Lanka |
Ravindra Satarasinghe | marjayawardana@yahoo.com |
| Serum ALT Is A Better Predictor Of Fibrosis Than Serum AST In Patients With NAFLD: Experience From A Tertiary Centre In Bangladesh |
Mamun Mahtab | hbd@dhaka.net |
| Aetiology Of Drug Induced Hepatitis: Experience From A Tertiary Centre In Bangladesh |
Mamun Mahtab | hbd@dhaka.net |
| Elevated expression of APRIL in hepatocellular carcinoma |
Wei Xiuqing | weixiuqing1975@yahoo.com.cn |
| Aetiology Of Non-Alcoholic Steatohepatitis: Experience From A Tertiary Centre In Bangladesh |
Mamun Mahtab | hbd@dhaka.net |
| Elevated expression of toll-like receptors 4 in hepatocellular carcinoma. |
Wei Xiuqing | weixiuqing1975@yahoo.com.cn |
| Insulin Resistance Is Not Frequently Association With Non-Alcoholic Steatohepatitis: Experience From A Tertiary Centre In Bangladesh |
Mamun Mahtab | hbd@dhaka.net |
| Insulin Sensitivity Index Is Superior To Insulin Resistance In Predicting Nash |
Mamun Mahtab | hbd@dhaka.net |
| Prevalence Of Non-Alcoholic Steatohepatitis In Patients With Non-Alcoholic Fatty Liver Disease: Experience From A Tertiary Centre In Bangladesh |
Mamun Mahtab | hbd@dhaka.net |
| Serum ALT Level Poorly Correlates With Necro-Inflammation And Fibrosis In Patients With NAFLD: Experience From A Tertiary Centre In Bangladesh |
Mamun Mahtab | hbd@dhaka.net |
| PROTECTIVE NUTRAGENOMIC EFFECT OF A PHYTOCOMPOUND ON OXIDATIVE STRESS AND DNA FRAGMENTATION AGAINST PARACETAMOL-INDUCED LIVER DAMAGE |
Francesco marotta | fmarchimede@libero.it |
| Lympidem® (a coumarin derivative) induced reversible hepatotoxicity in an adult Sri Lankan - A case report with literature review |
Ravindra Satarasinghe | ravindrasatharasinghe@yahoo.com |
| Wilson’s Disease- Is Penicillamine on the way out? |
Amitabh Monga | Amitabh_Monga@ttsh.com.sg |
| Analysis of Associated Factors and Frequency of Abnormal ALT in Nonalcoholic Fatty Liver Disease |
Suk Bae Kim | sm1213@paran.com |
| Epidemiology of bacteremic pyogenic liver abscess in Japan |
Goh Ohji | ohji@sanynet.ne.jp |
| A Pilot Study Utilizing Nitazoxanide for Hepatic Encephalopathy in Chronic Liver Failure |
Krishna Rayapudi | patbasumd@aol.com |
| A Retrospective Analysis Of The Safety Of Outpatient Percutaneous Liver Biopsies With Von Willebrand Disease |
Krishna Rayapudi | patbasumd@aol.com |
| Clinical-morphological changes of a liver at patients with insulin resistance |
Elena Gennadievna Egorova | toky0@mail.ru |
| Clinical observation on adjuvant therapy with gemcitabine and oxaliplatin for the patients with advanced liver cancer |
lin ma | linma001119@163.com |
| hepatitis B viral reactivation in rituximab-containing regimen for lymphoma |
Sang Hee Kim | biggystar@hanmail.net |
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